Fluorescence resonance energy transfer using DNA-templated copper nanoparticles for ratiometric detection of microRNAs

被引：18
作者：
Kim, Seokjoon [1 ]
Park, Ki Soo [1 ]
机构：
[1] Konkuk Univ, Coll Engn, Dept Biol Engn, Seoul 05029, South Korea
来源：
ANALYST | 2021年 / 146卷 / 06期
关键词：
NANOCLUSTERS;
D O I：
10.1039/d0an02371j
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We examined the effectiveness of a ratiometric method using DNA-templated copper nanoparticles, which can function as a probe for fluorescence resonance energy transfer. This method in combination with PCR successfully detected the target microRNA, which corresponded well with the results obtained by quantitative reverse transcription PCR.
引用
收藏
页码：1844 / 1847
页数：4
相关论文
共 22 条
[1]   Real-time quantification of microRNAs by stem-loop RT-PCR [J].
Chen, CF ;
Ridzon, DA ;
Broomer, AJ ;
Zhou, ZH ;
Lee, DH ;
Nguyen, JT ;
Barbisin, M ;
Xu, NL ;
Mahuvakar, VR ;
Andersen, MR ;
Lao, KQ ;
Livak, KJ ;
Guegler, KJ .
NUCLEIC ACIDS RESEARCH, 2005, 33 (20) :e179.1-e179.9
[2]   Switch-conversional ratiometric fluorescence biosensor for miRNA detection [J].
Chen, Xian ;
Xu, Ke ;
Li, Jing ;
Yang, Ming ;
Li, Xing ;
Chen, Qin ;
Lu, Chunhua ;
Yang, Huanghao .
BIOSENSORS & BIOELECTRONICS, 2020, 155
[3]   Nucleic Acid Sandwich Hybridization Assay with Quantum Dot-Induced Fluorescence Resonance Energy Transfer for Pathogen Detection [J].
Chou, Cheng-Chung ;
Huang, Yi-Han .
SENSORS, 2012, 12 (12) :16660-16672
[4]   Recent advances in the analytical applications of copper nanoclusters [J].
Hu, Xue ;
Liu, Tingting ;
Zhuang, Yunxia ;
Wang, Wei ;
Li, Yinying ;
Fan, Wenhong ;
Huang, Yuming .
TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2016, 77 :66-75
[5]   A facile "click" reaction to fabricate a FRET-based ratiometric fluorescent Cu2+ probe [J].
Hu, Zhangjun ;
Hu, Jiwen ;
Cui, Yang ;
Wang, Guannan ;
Zhang, Xuanjun ;
Uvdal, Kajsa ;
Gao, Hong-Wen .
JOURNAL OF MATERIALS CHEMISTRY B, 2014, 2 (28) :4467-4472
[6]   Plasma microRNAs are promising novel biomarkers for early detection of colorectal cancer [J].
Huang, Zhaohui ;
Huang, Dan ;
Ni, Shujuan ;
Peng, Zhilei ;
Sheng, Weiqi ;
Du, Xiang .
INTERNATIONAL JOURNAL OF CANCER, 2010, 127 (01) :118-126
[7]   Synthesis of DNA-templated copper nanoparticles with enhanced fluorescence stability for cellular imaging [J].
Kim, Seokjoon ;
Kim, Jung Ho ;
Kwon, Woo Young ;
Hwang, Sung Hyun ;
Cha, Byung Seok ;
Kim, Ji Min ;
Oh, Seung Soo ;
Park, Ki Soo .
MICROCHIMICA ACTA, 2019, 186 (07)
[8]   Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon [J].
Liu, Qingxin ;
Fan, Jialong ;
Zhou, Chuang ;
Wang, Liqun ;
Zhao, Bin ;
Zhang, Haibin ;
Liu, Bin ;
Tong, Chunyi .
INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY, 2018, 2018
[9]   DNA-templated copper nanoparticles: Versatile platform for label-free bioassays [J].
Liu, Rui ;
Wang, Chaoqun ;
Hu, Jianyu ;
Su, Yingying ;
Lv, Yi .
TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2018, 105 :436-452
[10]   Copper nanoclusters: Synthesis, characterization and properties [J].
Lu YiZhong ;
Wei WenTao ;
Chen Wei .
CHINESE SCIENCE BULLETIN, 2012, 57 (01) :41-47
123