Relations of lysophosphatidylcholine in low-density lipoprotein with serum lipoprotein-associated phospholipase A2, paraoxonase and homocysteine thiolactonase activities in patients with type 2 diabetes mellitus

被引：20

作者：

Sonoki, Kazuo ^{[1
]}

Iwase, Masanori ^{[1
]}

Sasaki, Nobuhiro ^{[1
]}

Ohdo, Shigehiro ^{[3
]}

Higuchi, Shun ^{[3
]}

Matsuyama, Naoto ^{[2
]}

Iida, Mitsuo ^{[1
]}

机构：

[1] Kyushu Univ, Dept Med & Clin Sci, Grad Sch Med Sci, Higashi Ku, Fukuoka 8128582, Japan

[2] Azwell Inc, R&D Div, Ibaraki, Japan

[3] Kyushu Univ, Grad Sch Pharmaceut Sci, Dept Clin Pharmacokinet, Fukuoka 8128582, Japan

来源：

DIABETES RESEARCH AND CLINICAL PRACTICE | 2009年 / 86卷 / 02期

关键词：

Lysophosphatidylcholine; Paraoxonase; Homocysteine thiolactonase; Lipoprotein-associated phospholipase A(2); Simvastatin; ACTIVATING-FACTOR ACETYLHYDROLASE; LDL MODIFICATION; MECHANISM; ATHEROSCLEROSIS; COMPLICATIONS; PEROXIDATION; INHIBITION; HYDROLASE; CAPACITY; THERAPY;

D O I：

10.1016/j.diabres.2009.08.014

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Aims: We studied the relations of lysophosphatidylcholine (lyso-PC) in LDL with serum lipoprotein-associated phospholipase A(2) (Lp-PLA(2)), paraoxonase and homocysteine thiolactonase (HTLase) activities in patients with type 2 diabetes mellitus. Methods: Lyso-PC was measured by electrospray ionization-liquid chromatography/mass spectrometry. Paraoxonase and HTLase activities were measured with paraoxon and gamma-thiobutyrolactone as substrates, respectively. Results: Serum HTLase and paraoxonase activities were significantly suppressed in diabetic patients (n = 96) compared with control (n = 25), whereas serum Lp-PLA(2) did not differ in control and diabetic patients. Lyso-PC contents in LDL correlated with serum Lp-PLA(2) activity positively and with serum HTLase activity negatively. Stepwise regression analysis revealed that serum Lp-PLA(2) and HTLase activities independently contributed to lyso-PC contents in LDL. In patients with diabetic nephropathy, lyso-PC contents in LDL were increased with reduced serum HTLase and paraoxonase activities compared with control, while serum Lp-PLA(2) activity did not differ. On the other hand, 3-month treatment with simvastatin reduced both lyso-PC contents in LDL and serum Lp-PLA(2) activity in hypercholesterolemic diabetic patients, while serum HTLase or paraoxonase activities did not change. Conclusions: Increased lyso-PC contents in LDL were associated with the suppressed HTLase activity, and serum Lp-PLA(2) and HTLase activities may be related to lyso-PC in type 2 diabetic patients. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：117 / 123

页数：7

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