Single-stage cell-based cartilage repair in a rabbit model: cell tracking and in vivo chondrogenesis of human umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel composite

被引：62

作者：

Park, Y. B. ^{[1
]}

Ha, C. W. ^{[2
,3
,4
]}

Kim, J. A. ^{[2
,3
]}

Han, W. J. ^{[2
,3
]}

Rhim, J. H. ^{[2
,3
]}

Lee, H. J. ^{[1
]}

Kim, K. J. ^{[5
]}

Park, Y. G. ^{[5
]}

Chung, J. Y. ^{[6
]}

机构：

[1] Chung Ang Univ, Chung Ang Univ Hosp, Coll Med, Dept Orthoped Surg, Seoul, South Korea

[2] Sungkyunkwan Univ, Samsung Med Ctr, Sch Med, Dept Orthoped Surg, 81 Irwon Ro, Seoul 06351, South Korea

[3] Sungkyunkwan Univ, Samsung Med Ctr, Sch Med, Stem Cell & Regenerat Med Inst, 81 Irwon Ro, Seoul 06351, South Korea

[4] Sungkyunkwan Univ, SAIHST, Dept Hlth Sci & Technol, Seoul, South Korea

[5] Jeju Natl Univ, Jeju Natl Univ Hosp, Sch Med, Dept Orthoped Surg, Jeju, South Korea

[6] Ajou Univ, Ajou Univ Hosp, Sch Med, Dept Orthoped Surg, Suwon, South Korea

来源：

OSTEOARTHRITIS AND CARTILAGE | 2017年 / 25卷 / 04期

关键词：

Cartilage repair; Cell transplantation; Mesenchymal stem cells; Human umbilical cord blood; Hyaluronic acid; Paracrine action; BONE-MARROW; ADIPOSE-TISSUE; RAT MODEL; OSTEOCHONDRAL DEFECT; PROGENITOR CELLS; STROMAL CELLS; DIFFERENTIATION; OSTEOARTHRITIS; REGENERATION; KNEE;

D O I：

10.1016/j.joca.2016.10.012

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

Objective: Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained popularity as a promising cell source for regenerative medicine, but limited in vivo studies have reported cartilage repair. In addition, the roles of MSCs in cartilage repair are not well-understood. The purpose of this study was to investigate the feasibility of transplanting hUCB-MSCs and hyaluronic acid (HA) hydrogel composite to repair articular cartilage defects in a rabbit model and determine whether the transplanted cells persisted or disappeared from the defect site. Design: Osteochondral defects were created in the trochlear grooves of the knees. The hUCB-MSCs and HA composite was transplanted into the defect of experimental knees. Control knees were transplanted by HA or left untreated. Animals were sacrificed at 8 and 16 weeks post-transplantation and additionally at 2 and 4 weeks to evaluate the fate of transplanted cells. The repair tissues were evaluated by gross, histological and immunohistochemical analysis. Results: Transplanting hUCB-MSCs and HA composite resulted in overall superior cartilage repair tissue with better quality than HA alone or no treatment. Cellular architecture and collagen arrangement at 16 weeks were similar to those of surrounding normal articular cartilage tissue. Histological scores also revealed that cartilage repair in experimental knees was better than that in control knees. Immunohistochemical analysis with anti-human nuclear antibody confirmed that the transplanted MSCs disappeared gradually over time. Conclusion: Transplanting hUCB-MSCs and HA composite promote cartilage repair and interactions between hUCB-MSCs and host cells initiated by paracrine action may play an important role in cartilage repair. (C) 2016 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

引用

页码：570 / 580

页数：11

共 47 条

[1] Human mesenchymal stem cells modulate allogeneic immune cell responses [J].