Synthesis of a 13C-methyl-group-labeled methionine precursor as a useful tool for simplifying protein structural analysis by NMR spectroscopy

被引：56
作者：
Fischer, Michael
Kloiber, Karin
Haeusler, Johannes
Ledolter, Karin
Konrat, Robert
Schmid, Walther
机构：
[1] Univ Vienna, Dept Biomol Struct Chem, A-1030 Vienna, Austria
[2] Univ Vienna, Inst Organ Chem, A-1090 Vienna, Austria
来源：
CHEMBIOCHEM | 2007年 / 8卷 / 06期
关键词：
amino acids; isotopic labeling; NMR spectroscopy; SH2; domain;
D O I：
10.1002/cbic.200600551
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
(Chemical Equation Presented) Marked for life. For the characterization of larger proteins new NMR spectroscopy methods that focus on side-chain methyl groups have been developed by using selectively isotope-labeled precursor compounds. Here we report on the synthesis of a 13C-methyl-group- labeled methionine precursor on a preparative scale, and its incorporation into the SH2 domain of the protein PLC-γ1 (see scheme). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
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收藏
页码：610 / 612
页数：3
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