Modulation of heterochromatin protein 1 dynamics in primary mammalian cells

被引：242
作者：
Festenstein, R
Pagakis, SN
Hiragami, K
Lyon, D
Verreault, A
Sekkali, B
Kioussis, D
机构：
[1] Imperial Coll Sch Med, Div Med, CSC Gene Control Mech & Dis Grp, London W12 0NN, England
[2] Natl Inst Med Res, Confocal & Image Anal Lab, London NW7 1AA, England
[3] London Res Inst, Canc Res UK, Chromosome Dynam Lab, Potters Bar EN6 3LD, Herts, England
[4] Natl Inst Med Res, Div Mol Immunol, London NW7 1AA, England
来源：
SCIENCE | 2003年 / 299卷 / 5607期
关键词：
D O I：
10.1126/science.1078694
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Heterochromatin protein 1 (HP1beta), a key component of condensed DNA, is strongly implicated in gene silencing and centromeric cohesion. Heterochromatin has been considered a static structure, stabilizing crucial aspects of nuclear organization and prohibiting access to transcription factors. We demonstrate here, by fluorescence recovery after photobleaching, that a green fluorescent protein-HP1beta fusion protein is highly mobile within both the euchromatin and heterochromatin of ex vivo resting murine T cells. Moreover, T cell activation greatly increased this mobility, indicating that such a process may facilitate (hetero)chromatin remodeling and permit access of epigenetic modifiers and transcription factors to the many genes that are consequently derepressed.
引用
收藏
页码：719 / 721
页数：3
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