A beginner's guide to tissue clearing

被引：86

作者：

Ariel, Pablo ^{[1
]}

机构：

[1] Univ N Carolina, Dept Pathol & Lab Med, Microscopy Serv Lab, Chapel Hill, NC 27599 USA

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | 2017年 / 84卷

关键词：

Tissue clearing; Optical clearing; Whole organ imaging; Whole organism imaging; Organoids; BRAIN; RESOLUTION; FEATURES; CLARITY;

D O I：

10.1016/j.biocel.2016.12.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The last decade has seen a proliferation of tissue clearing methods that render large biological samples transparent and allow unprecedented three-dimensional views of enormous volumes of tissue. For a scientist wondering whether these methods will be useful to address their research problems, it can be bewildering to sort through the ever-increasing number of papers introducing new clearing methods. Here, I provide a concise summary for the novice describing what tissue clearing is, which research problems it can be applied to, how to decide on a clearing method, and where the field is headed in the future. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：35 / 39

页数：5

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