Coexpression of CD58 or CD48 with intercellular adhesion molecule 1 on target cells enhances adhesion of resting NK cells

被引:82
作者
Barber, DF [1 ]
Long, EO [1 ]
机构
[1] NIAID, Immunogenet Lab, NIH, Rockville, MD 20852 USA
关键词
D O I
10.4049/jimmunol.170.1.294
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The beta(2) integrin LFA-1 (CD11a/CD18) mediates adhesion of lymphocytes to cells expressing ICAM. The strength of this adhesion is regulated by different signals delivered by cytokines and chemokines, and by the TCR in the case of T cells. To determine the receptor-ligand interactions required for adhesion of resting NK cells, Drosophila cells expressing different combinations of ligands of human NK cell receptors were generated. Expression of ICAM-1 alone was sufficient for an adhesion of resting NK cells that was sensitive to inhibitors of src family kinase and of phosphatidylinositol 3-kinase. Binding of resting NK cells to solid-phase ICAM-1 showed similar signaling requirements. A pulse of either IL-2 or IL-15 to resting NK cells resulted in strongly enhanced, actin-dependent adhesion to insect cells expressing ICAM-1 alone. Coexpression of either LFA-3 (CD58) or CD48 with ICAM-1 resulted in strong adhesion by resting NK cells, even in the absence of cytokines. Therefore, receptors for LFA-3 and CD48 on resting NK cells strengthen the adhesion mediated by LFA-1.
引用
收藏
页码:294 / 299
页数:6
相关论文
共 26 条
[21]  
2-I
[22]   TRAFFIC SIGNALS FOR LYMPHOCYTE RECIRCULATION AND LEUKOCYTE EMIGRATION - THE MULTISTEP PARADIGM [J].
SPRINGER, TA .
CELL, 1994, 76 (02) :301-314
[23]   LFA-1-mediated adhesion is regulated by cytoskeletal restraint and by a Ca2+-dependent protease, calpain [J].
Stewart, MP ;
McDowall, A ;
Hogg, N .
JOURNAL OF CELL BIOLOGY, 1998, 140 (03) :699-707
[24]   PARTICIPATION OF CD11A-C/CD18, CD2 AND RGD-BINDING RECEPTORS IN ENDOGENOUS AND INTERLEUKIN-2-STIMULATED NK ACTIVITY OF CD3-NEGATIVE LARGE ANTIGRANULOCYTES LYMPHOCYTES [J].
TIMONEN, T ;
GAHMBERG, CG ;
PATARROYO, M .
INTERNATIONAL JOURNAL OF CANCER, 1990, 46 (06) :1035-1040
[25]   Avidity regulation of integrins: the driving force in leukocyte adhesion [J].
van Kooyk, Y ;
Figdor, CG .
CURRENT OPINION IN CELL BIOLOGY, 2000, 12 (05) :542-547
[26]  
VOLTARELLI JC, 1993, IMMUNOLOGY, V79, P509