Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells

被引：54

作者：

Kojima, Kanako ^{[1
]}

Kuramochi-Miyagawa, Satomi ^{[1
,2
]}

Chuma, Shinichiro ^{[3
,4
]}

Tanaka, Takashi ^{[3
,4
]}

Nakatsuji, Norio ^{[3
,4
]}

Kimura, Tohru ^{[1
]}

Nakano, Toru ^{[1
]}

机构：

[1] Osaka Univ, Sch Med, Dept Pathol, Suita, Osaka 5650871, Japan

[2] JST, PREST, Kawaguchi, Saitama, Japan

[3] Kyoto Univ, Inst Frontier Med Sci, Sakyo Ku, Kyoto 6068507, Japan

[4] Kyoto Univ, Grad Sch Med, Sakyo Ku, Kyoto 6068507, Japan

来源：

GENES TO CELLS | 2009年 / 14卷 / 10期

基金：

日本学术振兴会;

关键词：

SMALL RNAS; INTRACELLULAR-LOCALIZATION; MICRORNA PATHWAY; DROSOPHILA-VASA; MOUSE MAELSTROM; FAMILY-MEMBERS; SELF-RENEWAL; HOMOLOG; TUDOR; GENE;

D O I：

10.1111/j.1365-2443.2009.01342.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The mouse Piwi family proteins (MILI, MIWI and MIWI2) play pivotal roles in spermatogenesis through transcriptional and post-transcriptional gene regulation. To reveal the molecular functions of these proteins, we investigate the proteins that bind to MILI in adult mouse testes. We found that both MILI and MIWI bind to TDRD1/MTR-1, which is also an essential protein for spermatogenesis. Co-immunoprecipitation assays and subcellular localization of the proteins and mutants thereof revealed a complex formation involving MILI, MIWI and TDRD1/MTR-1. In addition, the subcellular localizations of MILI and TDRD1/MTR-1 were altered, and chromatoid body formation was impaired in the MIWI-null round spermatids. These data suggest that the formation of complexes between MILI, MIWI and TDRD1/MTR-1 is critical for the integrated subcellular localizations of these proteins, and is presumably essential for spermatogenesis.

引用

页码：1155 / 1165

页数：11

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