Stenocarpella maydis and Stenocarpella macrospora species causing leaf spots and stem and ear rots, can be transported and disseminated between cultivating areas through seeds. The objective was to transform isolates of species of Stenocarpella with GFP and DsRed and to correlate different inoculum potentials with the effect caused by the presence of these pathogens in the tissues of maize seeds. The isolates were transformed with introduction of the genes in their nuclei, employing the technique of protoplast transformation. Seeds were inoculated by osmotic conditioning method with transformed and not transformed isolates, with different periods of exposition of seeds to those isolates, characterizing the inoculum potentials, P1 (24 h), P2 (48 h), P3 (72 h) and P4 (96 h). The seeds inoculated with isolates expressing GFP and DsRed in both species elucidated by means of the intensities of the emitted fluorescence, the ability of those organisms to cause infection and colonization in different inoculum potentials. The potentials P3 and P4 caused the highest levels of emitted fluorescence for the colonization by both pathogens. A comprehensive and abundant mycelial growth in the colonized seed structures were well visualized at potential P3 and P4 by means of SEM.
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Univ Kebangsaan Malaysia, Fac Sci & Technol, Dept Biol Sci & Biotechnol, Bangi 43600, Selangor, MalaysiaUniv Kebangsaan Malaysia, Fac Sci & Technol, Dept Biol Sci & Biotechnol, Bangi 43600, Selangor, Malaysia
Asyikha, Rosha
Mohd-Taib, Farah Shafawati
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Univ Kebangsaan Malaysia, Fac Sci & Technol, Dept Biol Sci & Biotechnol, Bangi 43600, Selangor, MalaysiaUniv Kebangsaan Malaysia, Fac Sci & Technol, Dept Biol Sci & Biotechnol, Bangi 43600, Selangor, Malaysia
Mohd-Taib, Farah Shafawati
Ishak, Siti Nabilah
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Univ Kebangsaan Malaysia, Fac Sci & Technol, Dept Biol Sci & Biotechnol, Bangi 43600, Selangor, MalaysiaUniv Kebangsaan Malaysia, Fac Sci & Technol, Dept Biol Sci & Biotechnol, Bangi 43600, Selangor, Malaysia
Ishak, Siti Nabilah
Jing, Khoo Jing
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Univ Malaya, Trop Infect Dis Res & Educ Ctr TIDREC, Kuala Lumpur 50603, MalaysiaUniv Kebangsaan Malaysia, Fac Sci & Technol, Dept Biol Sci & Biotechnol, Bangi 43600, Selangor, Malaysia
Jing, Khoo Jing
Sulaiman, Norela
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Univ Kebangsaan Malaysia, Fac Sci & Technol, Dept Biol Sci & Biotechnol, Bangi 43600, Selangor, MalaysiaUniv Kebangsaan Malaysia, Fac Sci & Technol, Dept Biol Sci & Biotechnol, Bangi 43600, Selangor, Malaysia