Single multiplex a polymerase chain reaction to detect diverse loci associated with a diarrheagenic Escherichia coli

被引：153

作者：

López-Saucedo, C

Cerna, JF

Villegas-Sepulveda, N

Thompson, R

Velazquez, FR

Torres, J

Tarr, PI

Estrada-García, T ^{[1
]}

机构：

[1] CINVESTAV IPN, Dpto Biomed Mol, Mexico City, DF, Mexico

[2] IMSS, Ctr Med Nacl Siglo XXI, Hosp Pediat, UIM Enfermedades Infecciosas & Parasitarias, Mexico City, DF, Mexico

[3] Washington Univ, Sch Med, Childrens Hosp, Seattle, WA USA

[4] Washington Univ, Sch Med, Reg Med Ctr, Seattle, WA USA

来源：

EMERGING INFECTIOUS DISEASES | 2003年 / 9卷 / 01期

关键词：

D O I：

10.3201/eid0901.010507

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We developed and tested a single multiplex polymerase chain reaction (PCR) that detects enterotoxigenic enteropathogenic, enteroinvasive, and Shiga toxin-producing Escherichia coli. This PCR is specific, sensitive. and rapid in detecting target isolates in stool and food, Because of its simplicity, economy, and efficiency, this protocol warrants further evaluation in large, prospective studies of polymicrobial substances.

引用

页码：127 / 131

页数：5

共 27 条

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