An open-access microfluidic model for lung-specific functional studies at an air-liquid interface

被引：72

作者：

Nalayanda, Divya D. ^{[1
,2
]}

Puleo, Christopher ^{[2
]}

Fulton, William B. ^{[1
]}

Sharpe, Leilani M. ^{[1
]}

Wang, Tza-Huei ^{[2
,3
]}

Abdullah, Fizan ^{[1
]}

机构：

[1] Johns Hopkins Med Inst, Div Pediat Surg, Baltimore, MD 21287 USA

[2] Johns Hopkins Univ, Dept Biomed Engn, Baltimore, MD USA

[3] Johns Hopkins Univ, Dept Mech Engn, Baltimore, MD 21218 USA

来源：

BIOMEDICAL MICRODEVICES | 2009年 / 11卷 / 05期

关键词：

Microfluidic cell culture; Alveolar cells; Air-liquid interface; MYCOBACTERIUM-TUBERCULOSIS; CELL; CULTURE;

D O I：

10.1007/s10544-009-9325-5

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In an effort to improve the physiologic relevance of existing in vitro models for alveolar cells, we present a microfluidic platform which provides an air-interface in a dynamic system combining microfluidic and suspended membrane culture systems. Such a system provides the ability to manipulate multiple parameters on a single platform along with ease in cell seeding and manipulation. The current study presents a comparison of the efficacy of the hybrid system with conventional platforms using assays analyzing the maintenance of function and integrity of A549 alveolar epithelial cell monolayer cultures. The hybrid system incorporates bio-mimetic nourishment on the basal side of the epithelial cells along with an open system on the apical side of the cells exposed to air allowing for easy access for assays.

引用

页码：1081 / 1089

页数：9

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