Atopobium vaginae and Prevotella bivia Are Able to Incorporate and Influence Gene Expression in a Pre-Formed Gardnerella vaginalis Biofilm

被引:40
作者
Castro, Joana [1 ]
Rosca, Aliona S. [1 ]
Muzny, Christina A. [2 ]
Cerca, Nuno [1 ]
机构
[1] Univ Minho, Ctr Biol Engn CEB, Lab Res Biofilms Rosario Oliveira LIBRO, Campus Gualtar, P-4710057 Braga, Portugal
[2] Univ Alabama Birmingham, Div Infect Dis, Birmingham, AL 35294 USA
关键词
bacterial vaginosis; Gardnerella spp; Atopobium vaginae; Prevotella bivia; polymicrobial biofilms; virulence; BACTERIAL-VAGINOSIS; CYTOLYTIC ACTIVITY; LACTOBACILLUS; PATHOGENESIS; ACQUISITION; VAGINOLYSIN; DIVERSITY; INFECTION; ADHESION; DEPENDS;
D O I
10.3390/pathogens10020247
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Bacterial vaginosis (BV) is associated with a highly structured polymicrobial biofilm on the vaginal epithelium where Gardnerella species presumably play a pivotal role. Gardnerella vaginalis, Atopobium vaginae, and Prevotella bivia are vaginal pathogens detected during the early stages of incident BV. Herein, we aimed to analyze the impact of A. vaginae and P. bivia on a pre-established G. vaginalis biofilm using a novel in vitro triple-species biofilm model. Total biofilm biomass was determined by the crystal violet method. We also discriminated the bacterial populations in the biofilm and in its planktonic fraction by using PNA FISH. We further analyzed the influence of A. vaginae and P. bivia on the expression of key virulence genes of G. vaginalis by quantitative PCR. In our tested conditions, A. vaginae and P. bivia were able to incorporate into pre-established G. vaginalis biofilms but did not induce an increase in total biofilm biomass, when compared with 48-h G. vaginalis biofilms. However, they were able to significantly influence the expression of HMPREF0424_0821, a gene suggested to be associated with biofilm maintenance in G. vaginalis. This study suggests that microbial relationships between co-infecting bacteria can deeply affect the G. vaginalis biofilm, a crucial marker of BV.
引用
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页码:1 / 16
页数:15
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