Cytokines and Signaling Pathways Regulating Matrix Metalloproteinase-9 (MMP-9) Expression in Corneal Epithelial Cells
被引:86
作者:
Gordon, Gabriel M.
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机构:
Univ So Calif, Keck Sch Med, Inst Med Genet, Los Angeles, CA 90033 USA
Univ Miami, Miller Sch Med, Bascom Palmer Eye Inst, Miami, FL 33136 USAUniv So Calif, Keck Sch Med, Inst Med Genet, Los Angeles, CA 90033 USA
Gordon, Gabriel M.
[1
,2
]
Ledee, Dolena R.
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机构:
Univ Miami, Miller Sch Med, Bascom Palmer Eye Inst, Miami, FL 33136 USAUniv So Calif, Keck Sch Med, Inst Med Genet, Los Angeles, CA 90033 USA
Ledee, Dolena R.
[2
]
Feuer, William J.
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机构:
Univ Miami, Miller Sch Med, Bascom Palmer Eye Inst, Miami, FL 33136 USAUniv So Calif, Keck Sch Med, Inst Med Genet, Los Angeles, CA 90033 USA
Feuer, William J.
[2
]
Fini, M. Elizabeth
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h-index: 0
机构:
Univ So Calif, Keck Sch Med, Inst Med Genet, Los Angeles, CA 90033 USA
Univ Miami, Miller Sch Med, Bascom Palmer Eye Inst, Miami, FL 33136 USAUniv So Calif, Keck Sch Med, Inst Med Genet, Los Angeles, CA 90033 USA
Fini, M. Elizabeth
[1
,2
]
机构:
[1] Univ So Calif, Keck Sch Med, Inst Med Genet, Los Angeles, CA 90033 USA
[2] Univ Miami, Miller Sch Med, Bascom Palmer Eye Inst, Miami, FL 33136 USA
Matrix metalloproteinase-9 (MMP-9) is a well-known regulator and effecter of many cellular processes including wound healing. In the cornea, either too much or too little MMP-9 can be detrimental to overall wound repair. We investigated the secreted factors as well as the intracellular signaling pathways and the promoter sequences that mediate this regulation. Primary culture rabbit corneal epithelial cells were treated with various cytokines alone or in different combinations and MMP-9 induction was assessed by gel zymography. Pharmacological inhibitors were used to determine the intracellular signaling pathways induced by the cytokines tested and deletion promoter constructs were created to determine the regions of the MMP-9 promoter involved in the cytokine regulation, thereby assessing the exact transcription factors binding the MMP-9 promoter. We found that two cytokine families, transforming growth factor beta (TGF-beta) and interleukin 1 (IL-1), act additively in an isoform non-specific manner to induce MMP-9 in this cell type. Our data suggest TGF-beta mediated MMP-9 induction may be regulated by the NF-kappa B, Smad3 and JNK pathways, whereas the IL-1 beta mediated induction may be regulated by the NF-kappa B and p38 pathways. Inhibition of the p38, NF-kappa B, or JNK pathways significantly reduced, but did not abrogate, basal MMP-9 levels. Inhibition of the ERK pathway did not have an effect on MMP-9 mediated expression in either the treated or untreated co-transfected cells. J. Cell. Physiol. 221: 402-411, 2009. (C) 2009 Wiley-Liss, Inc.
机构:
Mahidol Univ, Fac Med, Dept Surg,Siriraj Hosp, Div Head & Neck Surg, Bangkok 10700, ThailandMahidol Univ, Fac Med, Dept Surg,Siriraj Hosp, Div Head & Neck Surg, Bangkok 10700, Thailand
O-Charoenrat, P
Wongkajornsilp, A
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机构:Mahidol Univ, Fac Med, Dept Surg,Siriraj Hosp, Div Head & Neck Surg, Bangkok 10700, Thailand
Wongkajornsilp, A
Rhys-Evans, PH
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机构:Mahidol Univ, Fac Med, Dept Surg,Siriraj Hosp, Div Head & Neck Surg, Bangkok 10700, Thailand
Rhys-Evans, PH
Eccles, SA
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h-index: 0
机构:Mahidol Univ, Fac Med, Dept Surg,Siriraj Hosp, Div Head & Neck Surg, Bangkok 10700, Thailand
机构:
Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Nguyen, Trung T.
Ding, Derong
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Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Ding, Derong
Wolter, William R.
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Univ Notre Dame, Freimann Life Sci Ctr, Notre Dame, IN 46556 USA
Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Wolter, William R.
Perez, Rocio L.
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Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Perez, Rocio L.
Champion, Matthew M.
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Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Champion, Matthew M.
Mahasenan, Kiran V.
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Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Mahasenan, Kiran V.
Hesek, Dusan
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h-index: 0
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Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Hesek, Dusan
Lee, Mijoon
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Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Lee, Mijoon
Schroeder, Valerie A.
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机构:
Univ Notre Dame, Freimann Life Sci Ctr, Notre Dame, IN 46556 USA
Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Schroeder, Valerie A.
Jones, Jeffrey I.
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Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Jones, Jeffrey I.
Lastochkin, Elena
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Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Lastochkin, Elena
Rose, Margaret K.
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Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Rose, Margaret K.
Peterson, Charles E.
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h-index: 0
机构:
Elkhart Gen Hosp, Ctr Wound Healing, Elkhart, IN 46514 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Peterson, Charles E.
Suckow, Mark A.
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h-index: 0
机构:
Univ Notre Dame, Freimann Life Sci Ctr, Notre Dame, IN 46556 USA
Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA
Univ Minnesota, Coll Vet Med, Vet Populat Med Dept, St Paul, MN 55108 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Suckow, Mark A.
Mobashery, Shahriar
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机构:
Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Mobashery, Shahriar
Chang, Mayland
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Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
机构:
Mahidol Univ, Fac Med, Dept Surg,Siriraj Hosp, Div Head & Neck Surg, Bangkok 10700, ThailandMahidol Univ, Fac Med, Dept Surg,Siriraj Hosp, Div Head & Neck Surg, Bangkok 10700, Thailand
O-Charoenrat, P
Wongkajornsilp, A
论文数: 0引用数: 0
h-index: 0
机构:Mahidol Univ, Fac Med, Dept Surg,Siriraj Hosp, Div Head & Neck Surg, Bangkok 10700, Thailand
Wongkajornsilp, A
Rhys-Evans, PH
论文数: 0引用数: 0
h-index: 0
机构:Mahidol Univ, Fac Med, Dept Surg,Siriraj Hosp, Div Head & Neck Surg, Bangkok 10700, Thailand
Rhys-Evans, PH
Eccles, SA
论文数: 0引用数: 0
h-index: 0
机构:Mahidol Univ, Fac Med, Dept Surg,Siriraj Hosp, Div Head & Neck Surg, Bangkok 10700, Thailand
机构:
Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Nguyen, Trung T.
Ding, Derong
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h-index: 0
机构:
Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Ding, Derong
Wolter, William R.
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机构:
Univ Notre Dame, Freimann Life Sci Ctr, Notre Dame, IN 46556 USA
Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Wolter, William R.
Perez, Rocio L.
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h-index: 0
机构:
Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Perez, Rocio L.
Champion, Matthew M.
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h-index: 0
机构:
Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Champion, Matthew M.
Mahasenan, Kiran V.
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h-index: 0
机构:
Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Mahasenan, Kiran V.
Hesek, Dusan
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h-index: 0
机构:
Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Hesek, Dusan
Lee, Mijoon
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h-index: 0
机构:
Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Lee, Mijoon
Schroeder, Valerie A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Notre Dame, Freimann Life Sci Ctr, Notre Dame, IN 46556 USA
Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Schroeder, Valerie A.
Jones, Jeffrey I.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Jones, Jeffrey I.
Lastochkin, Elena
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h-index: 0
机构:
Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Lastochkin, Elena
Rose, Margaret K.
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h-index: 0
机构:
Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Rose, Margaret K.
Peterson, Charles E.
论文数: 0引用数: 0
h-index: 0
机构:
Elkhart Gen Hosp, Ctr Wound Healing, Elkhart, IN 46514 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Peterson, Charles E.
Suckow, Mark A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Notre Dame, Freimann Life Sci Ctr, Notre Dame, IN 46556 USA
Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA
Univ Minnesota, Coll Vet Med, Vet Populat Med Dept, St Paul, MN 55108 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Suckow, Mark A.
Mobashery, Shahriar
论文数: 0引用数: 0
h-index: 0
机构:
Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
Mobashery, Shahriar
Chang, Mayland
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h-index: 0
机构:
Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USAUniv Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA