Cytokines and Signaling Pathways Regulating Matrix Metalloproteinase-9 (MMP-9) Expression in Corneal Epithelial Cells

被引:86
作者
Gordon, Gabriel M. [1 ,2 ]
Ledee, Dolena R. [2 ]
Feuer, William J. [2 ]
Fini, M. Elizabeth [1 ,2 ]
机构
[1] Univ So Calif, Keck Sch Med, Inst Med Genet, Los Angeles, CA 90033 USA
[2] Univ Miami, Miller Sch Med, Bascom Palmer Eye Inst, Miami, FL 33136 USA
关键词
NF-KAPPA-B; METALLOPROTEINASE GELATINASE-B; GROWTH-FACTOR-BETA; EXPERIMENTAL DRY EYE; MATRIX-METALLOPROTEINASE; TRANSCRIPTION FACTORS; P38; MAPK; COLLAGENASE SYNTHESIS; CEREBROSPINAL-FLUID; WOUND REPAIR;
D O I
10.1002/jcp.21869
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Matrix metalloproteinase-9 (MMP-9) is a well-known regulator and effecter of many cellular processes including wound healing. In the cornea, either too much or too little MMP-9 can be detrimental to overall wound repair. We investigated the secreted factors as well as the intracellular signaling pathways and the promoter sequences that mediate this regulation. Primary culture rabbit corneal epithelial cells were treated with various cytokines alone or in different combinations and MMP-9 induction was assessed by gel zymography. Pharmacological inhibitors were used to determine the intracellular signaling pathways induced by the cytokines tested and deletion promoter constructs were created to determine the regions of the MMP-9 promoter involved in the cytokine regulation, thereby assessing the exact transcription factors binding the MMP-9 promoter. We found that two cytokine families, transforming growth factor beta (TGF-beta) and interleukin 1 (IL-1), act additively in an isoform non-specific manner to induce MMP-9 in this cell type. Our data suggest TGF-beta mediated MMP-9 induction may be regulated by the NF-kappa B, Smad3 and JNK pathways, whereas the IL-1 beta mediated induction may be regulated by the NF-kappa B and p38 pathways. Inhibition of the p38, NF-kappa B, or JNK pathways significantly reduced, but did not abrogate, basal MMP-9 levels. Inhibition of the ERK pathway did not have an effect on MMP-9 mediated expression in either the treated or untreated co-transfected cells. J. Cell. Physiol. 221: 402-411, 2009. (C) 2009 Wiley-Liss, Inc.
引用
收藏
页码:402 / 411
页数:10
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