A quick and simple biostrip technique for detection of lactose

被引:12
作者
Sharma, SK
Sehgal, N
Kumar, A
机构
[1] Univ Delhi, Ctr Biochem Technol, Delhi 110007, India
[2] Univ Delhi, Dept Zool, Delhi 110007, India
关键词
beta-galactosidase; biostrip; lactase; lactose; lactose intolerance;
D O I
10.1023/A:1020601400616
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A quick, simple and economical biostrip technology was developed for estimation of lactose by immobilizing galactosidase, galactose oxidase and horseradish peroxidase on to a polymeric support. The biostrip is dipped in milk or milk products and, from the colour that develops from an added chromogen, the concentration of lactose can be estimated from <20 to 100+g l(-1). The biostrips may be used in dairy industries, hospitals and remote areas where expensive instruments are not available.
引用
收藏
页码:1737 / 1739
页数:3
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