Inhibitory Mechanism of Engeletin Against α-Glucosidase

被引：3

作者：

Li, Yunbo ^{[1
,2
]}

Liu, Xiaoling ^{[1
]}

Zhou, Haoyu ^{[1
]}

Li, Bo ^{[1
]}

Mazurenko, Igor Kostiantinovich ^{[2
]}

机构：

[1] Henan Inst Sci & Technol, Sch Food Sci, Xinxiang 453003, Henan, Peoples R China

[2] Sumy Natl Agr Univ, Dept Food Technol, Sumy, Ukraine

来源：

NATURAL PRODUCT COMMUNICATIONS | 2021年 / 16卷 / 01期

关键词：

engeletin; alpha-glucosidase; inhibition; fluorescence spectroscopy; molecular docking;

D O I：

10.1177/1934578X20986723

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The inhibitory mechanism of engeletin against a-glucosidase was investigated for the first time by fluorescence spectroscopy and molecular docking. The results showed that engeletin could inhibit alpha-glucosidase in a noncompetitive inhibition mode with a half-maximal inhibitory concentration value of 48.5 +/- 6.0 mu g/mL (0.11 +/- 0.014 mmol/L). It was found that engeletin could cause static fluorescence quenching of alpha-glucosidase by forming a complex with alpha-glucosidase. The thermodynamic parameters indicated that the combination of engeletin and alpha-glucosidase was driven by hydrophobic force. The molecular docking results confirmed that some amino acid residues of alpha-glucosidase (Trp391, Arg428, Glu429, Gly566, Trp710, Glu771) could interact with engeletin by hydrogen bonding.

引用

页数：5

共 17 条

[1] Angiotensin-Converting Enzyme Inhibitory Effects by Plant Phenolic Compounds: A Study of Structure Activity Relationships [J].

Al Shukor, Nadin ;

Van Camp, John ;

Gonzales, Gerard Bryan ;

Staljanssens, Dorien ;

Struijs, Karin ;

Zotti, Moises J. ;

Raes, Katleen ;

Smagghe, Guy .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2013, 61 (48) :11832-11839

[2] Engeletin suppresses cervical carcinogenesis in vitro and in vivo by reducing NF-κB-dependent signaling [J].

Bai, Hongwei ;

Yin, Haiqin .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2020, 526 (02) :497-504

[3]

Coman C, 2012, NOT BOT HORTI AGROBO, V40, P314

[4] α- and β-Glucosidase inhibitors:: chemical structure and biological activity [J].

de Melo, Eduardo Borges ;

Gomes, Adriane da Silveira ;

Carvalho, Ivone .

TETRAHEDRON, 2006, 62 (44) :10277-10302

[5] Modulation of Starch Digestion for Slow Glucose Release through "Toggling" of Activities of Mucosal α-Glucosidases [J].

Lee, Byung-Hoo ;

Eskandari, Razieh ;

Jones, Kyra ;

Reddy, Kongara Ravinder ;

Quezada-Calvillo, Roberto ;

Nichols, Buford L. ;

Rose, David R. ;

Hamaker, Bruce R. ;

Pinto, B. Mario .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (38) :31929-31938

[6] Comparative Evaluation of Quercetin, Isoquercetin and Rutin as Inhibitors of α-Glucosidase [J].

Li, Yan Qin ;

Zhou, Feng Chao ;

Gao, Fei ;

Bian, Jun Sheng ;

Shan, Fang .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2009, 57 (24) :11463-11468

[7] A class of novel AA-Trp-Trp-OBzl: synthesis, in vitro anti-proliferation, in vivo anti-tumor action, and intercalation mechanism [J].

Liu, Liu ;

Wei, Lei ;

Yang, Yifan ;

Zhao, Ming ;

Zhang, Xiaoyi ;

Zheng, Meiqing ;

Wang, Yuji ;

Peng, Shiqi .

MEDCHEMCOMM, 2011, 2 (02) :126-131

[8] Engeletin suppresses lung cancer progression by inducing apoptotic cell death through modulating the XIAP signaling pathway: A molecular mechanism involving ER stress [J].

Liu, Ting ;

Li, Yang ;

Sun, Jiaolin ;

Tian, Gang ;

Shi, Zhihong .

BIOMEDICINE & PHARMACOTHERAPY, 2020, 128

[9] A spectroscopic and thermal stability study on the interaction between putrescine and bovine trypsin [J].

Momeni, Lida ;

Shareghi, Behzad ;

Saboury, Ali A. ;

Farhadian, Sadegh ;

Reisi, Fateme .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2017, 94 :145-153

[10] Fruit, vegetables, and mushrooms for the preparation of extracts with α-amylase and α-glucosidase inhibition properties: A review [J].

Papoutsis, Konstantinos ;

Zhang, Jinyuan ;

Bowyer, Michael C. ;

Brunton, Nigel ;

Gibney, Eileen R. ;

Lyng, James .

FOOD CHEMISTRY, 2021, 338

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