Phytohormone production by three strains of Bradyrhizobium japonicum and possible physiological and technological implications

被引:145
作者
Boiero, L.
Perrig, D.
Masciarelli, O.
Penna, C.
Cassan, F.
Luna, V.
机构
[1] Univ Nacl Rio Cuarto, Dept Ciencias Nat, Lab Fisiol Vegetal, RA-5800 Rio Cuarto, Argentina
[2] Nitragin Argentina SA, Dept Invest & Desarrollo, Buenos Aires, DF, Argentina
关键词
D O I
10.1007/s00253-006-0731-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The aim of this work was to evaluate phytohormone biosynthesis, siderophores production, and phosphate solubilization in three strains (E109, USDA110, and SEMIA5080) of Bradyrhizobium japonicum, most commonly used for inoculation of soybean and nonlegumes in USA, Canada, and South America. Siderophore production and phosphate solubilization were evaluated in selective culture conditions, which had negative results. Indole-3-acetic acid (IAA), gibberellic acid (GA(3)), and abscisic acid (ABA) production were analyzed by gas chromatography-mass spectrometry (GC-MS). Ethylene and zeatin biosynthesis were determined by GS-flame ionization detection and high-performance liquid chromatography (HPLC-UV), respectively. IAA, zeatin, and GA(3) were found in all three strains; however, their levels were significantly higher (p < 0.01) in SEMIA5080 (3.8 mu g ml(-1)), USDA110 (2.5 mu g ml(-1)), and E109 (0.87 mu g ml(-1)), respectively. ABA biosynthesis was detected only in USDA110 (0.019 mu g ml(-1)). Ethylene was found in all three strains, with highest production rate (18.1 ng ml(-1) h(-1)) in E109 cultured in yeast extract mannitol medium plus L-methionine. This is the first report of IAA, GA(3), zeatin, ethylene, and ABA production by B. japonicum in pure cultures, using quantitative physicochemical methodology. The three strains have differential capability to produce the five major phytohormones and this fact may have an important technological implication for inoculant formulation.
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页码:874 / 880
页数:7
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