Targeting antigens to Dec-205 on dendritic cells induces a higher immune response in chickens: Hemagglutinin of avian influenza virus example

被引：23

作者：

Jauregui-Zuniga, David ^{[1
]}

Pedraza-Escalona, Martha ^{[1
]}

Pavel Espino-Solis, Gerardo ^{[1
,3
]}

Quintero-Hernandez, Veronica ^{[1
]}

Olvera-Rodriguez, Alejandro ^{[1
]}

Aurelio Diaz-Salinas, Marco ^{[2
,4
]}

Lopez, Susana ^{[2
]}

Domingos Possani, Lourival ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Med Mol Bioproc, Apartado Postal 510-3, Cuernavaca 62210, Morelos, Mexico

[2] Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Apartado Postal 510-3, Cuernavaca 62210, Morelos, Mexico

[3] Baylor Inst Immunol Res, 3434 Live Oak St, Dallas, TX 75204 USA

[4] Inst Rech Clin Montreal IRCM, Lab Human Retrovirol, 110 Pine Ave West, Montreal, PQ H2W 1R7, Canada

来源：

RESEARCH IN VETERINARY SCIENCE | 2017年 / 111卷

关键词：

Antigen targeting; Avian influenza; DEC-205; Hemagglutinin; IN-VIVO; RECEPTOR DEC-205; ANTIBODY; PROTEIN; ENDOCYTOSIS; VACCINE; FUSION; MICE;

D O I：

10.1016/j.rvsc.2016.12.002

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

It is widely known that targeting a variety of antigens to the DEC-205 receptor on dendritic cells (DCs) significantly potentiate immunity. This communication reports the development of a new murine monoclonal antibody (mAb) against the chicken DEC-205, using as immunogen the carbohydrate recognition domain-2 (CRD-2) heterologously expressed. This mAb recognizes a protein band of 250 kDa by immunoprecipitation analysis and shows strong cross-reactivity with human and pig DEC-205. Furthermore, the hemagglutinin (HA) of avian influenza H5N2 virus was cloned and expressed using insect cell-baculovirus expression system. We chemically conjugated the anti-chicken DEC-205 antibody with the highly purified HA to direct the antigen to the dendritic cells and evaluate the immune response elicited in vivo by this conjugate. A single dose of chemical conjugate was sufficient to elicit a strong immune response in chickens as early as fourteen days after priming. In addition, the conjugate induced an earlier and higher response compared to unconjugated HA. These results suggest that the strategy described here has potential to be used in the future design and development of successful vaccines against different chicken infectious diseases with direct impact in biotechnology and veterinary fields. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：55 / 62

页数：8

共 28 条

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