Angiotensin II activates intermediate-conductance Ca2+-activated K+ channels in arterial smooth muscle cells

Angiostensin II (Ang II) regulates the migration and proliferation of vascular smooth muscle cells. Recent studies indicate that intermediate-conductance Ca2+-activated K+ (IKca) channels have an important role in cell migration and proliferation. It is not known, however, whether the action of Ang II is linked to IKca channel regulation. Here, we investigated the modulation of lKca channels by Ang II in artery smooth muscle cells. Functional IKca channel expression in cultured embryonic rat aorta smooth muscle (A 10) cells was studied using the patch-clamp technique. These cells predominantly express IKca channels. In contrast, large-conductance Ca2+-activated K+ (BKca) currents were rarely observed in excised patches. Ang II increased the lKca current in a contration-dependent manner. Losartan (1.0 mu M), an ATI selective antagonist, abolished the activation of IKca channels by Ang II. Pretreatment with 100 mu M myristoylated protein kinase C inhibitor peptide 20-28 or 10 mu M GF109203X completely abolished the AngII-induced activation of lKca currents, whereas the action of Ang II was not prevented in the presence of 100 mu M Rp-cyclic 3', 5'hydrogen phosphotiate adenosine triethylammonium, a protein kinase A inhibitor, or 1.0 mu M KT-5823, a protein kinase G inhibitor. A membrane permeant analogue of diacylglycerol 1, 2-dioctanoyl-sn-glycerol (10 mu M) induced the activation of IKca currents. These data suggest that Ang II activates IKca channels through the activation of protein kinase C, and the AT1 receptor is involved in the regulation of these channels. (C) 2006 Elsevier Inc. All rights reserved.