Proteins that underlie neoplastic progression of ulcerative colitis

被引:42
作者
Brentnall, Teresa A.
Pan, Sheng [2 ]
Bronner, Mary P. [3 ]
Crispin, David A.
Mirzaei, Hamid [4 ]
Cooke, Kelly [4 ]
Tamura, Yasuko
Nikolskaya, Tatiana [5 ,6 ]
JeBailey, Lellean [6 ]
Goodlett, David R. [7 ]
McIntosh, Martin [8 ]
Aebersold, Ruedi [4 ,9 ,10 ]
Rabinovitch, Peter S. [2 ]
Chen, Ru [1 ]
机构
[1] Univ Washington, Dept Med, Div Gastroenterol, Seattle, WA 98195 USA
[2] Univ Washington, Dept Pathol, Seattle, WA 98195 USA
[3] Cleveland Clin Fdn, Dept Anat Pathol, Cleveland, OH 44195 USA
[4] Inst Syst Biol, Seattle, WA USA
[5] Russian Acad Sci, NI Vavilov Gen Genet Res Inst, Moscow, Russia
[6] GeneGo Inc, St Joseph, MI USA
[7] Univ Washington, Dept Med Chem, Seattle, WA 98195 USA
[8] Fred Hutchinson Canc Res Ctr, Mol Diagnost Program, Seattle, WA 98104 USA
[9] Univ Zurich, Fac Sci, CH-8006 Zurich, Switzerland
[10] Swiss Fed Inst Technol, Inst Mol Syst Biol, Zurich, Switzerland
关键词
Biomarker; Cancer; Dysplasia; Neoplastic progression; Ulcerative colitis; MITOCHONDRIAL-DNA MUTATIONS; C-MYC; CHROMOSOMAL INSTABILITY; INCREASED EXPRESSION; CANCER; PROTEOMICS; DYSPLASIA; S100P; QUANTITATION; ACTIVATION;
D O I
10.1002/prca.200900061
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Patients with ulcerative colitis (UC) have an increased risk for developing colorectal cancer. Because UC tumorigenesis is associated with genomic field defects that can extend throughout the entire colon, including the non-dysplastic mucosa, we hypothesized that the same field defects will include abnormally expressed proteins. Here, we applied proteomics to study the protein expression of UC neoplastic progression. The protein profiles of colonic epithelium were compared with (i) UC patients without dysplasia (non-progressors), (ii) non-dysplastic colonic tissue from UC patient with high-grade dysplasia or cancer (progressors), (iii) high-grade dysplastic tissue from UC progressors, and (iv) normal colon. We identified differential protein expression associated with UC neoplastic progression. Proteins relating to mitochondria, oxidative activity, and calcium-binding proteins were some of the interesting classes of these proteins. Network analysis discovered that Sp1 and c-myc proteins may play roles in UC early and late stages of neoplastic progression, respectively. Two over-expressed proteins in the non-dysplastic tissue of UC progressors, carbamoyl-phosphate synthase 1 and S100P, were further confirmed by immunohistochemistry analysis. Our study provides insight into the molecular events associated with UC neoplastic progression, which could be exploited for the development of protein biomarkers in fields of non-dysplastic mucosa that identify a patient's risk for UC dysplasia.
引用
收藏
页码:1326 / 1337
页数:12
相关论文
共 43 条
[31]   Accumulation of mitochondrial DNA mutation with colorectal carcinogenesis in ulcerative colitis [J].
Nishikawa, M ;
Oshitani, N ;
Matsumoto, T ;
Nishigami, T ;
Arakawa, T ;
Inoue, M .
BRITISH JOURNAL OF CANCER, 2005, 93 (03) :331-337
[32]   Chromosomal instability in ulcerative colitis is related to telomere shortening [J].
O'Sullivan, JN ;
Bronner, MP ;
Brentnall, TA ;
Finley, JC ;
Shen, WT ;
Emerson, S ;
Emond, MJ ;
Gollahon, KA ;
Moskovitz, AH ;
Crispin, DA ;
Potter, JD ;
Rabinovitch, PS .
NATURE GENETICS, 2002, 32 (02) :280-284
[33]  
PAVELIC ZP, 1992, ANTICANCER RES, V12, P171
[34]  
Rabinovitch PS, 1999, CANCER RES, V59, P5148
[35]   DYSPLASIA IN INFLAMMATORY BOWEL-DISEASE - STANDARDIZED CLASSIFICATION WITH PROVISIONAL CLINICAL-APPLICATIONS [J].
RIDDELL, RH ;
GOLDMAN, H ;
RANSOHOFF, DF ;
APPELMAN, HD ;
FENOGLIO, CM ;
HAGGITT, RC ;
AHREN, C ;
CORREA, P ;
HAMILTON, SR ;
MORSON, BC ;
SOMMERS, SC ;
YARDLEY, JH .
HUMAN PATHOLOGY, 1983, 14 (11) :931-968
[36]   Ulcerative colitis is a disease of accelerated colon aging: Evidence from telomere attrition and DNA damage [J].
Risques, Rosa Ana ;
Lai, Lisa A. ;
Brentnall, Teresa A. ;
Li, Lin ;
Feng, Ziding ;
Gallaher, Jasmine ;
Mandelson, Margaret T. ;
Potter, John D. ;
Bronner, Mary P. ;
Rabinovitch, Peter S. .
GASTROENTEROLOGY, 2008, 135 (02) :410-418
[37]   Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents [J].
Ross, PL ;
Huang, YLN ;
Marchese, JN ;
Williamson, B ;
Parker, K ;
Hattan, S ;
Khainovski, N ;
Pillai, S ;
Dey, S ;
Daniels, S ;
Purkayastha, S ;
Juhasz, P ;
Martin, S ;
Bartlet-Jones, M ;
He, F ;
Jacobson, A ;
Pappin, DJ .
MOLECULAR & CELLULAR PROTEOMICS, 2004, 3 (12) :1154-1169
[38]   DNA ANEUPLOIDY IN COLONIC BIOPSIES PREDICTS FUTURE-DEVELOPMENT OF DYSPLASIA IN ULCERATIVE-COLITIS [J].
RUBIN, CE ;
HAGGITT, RC ;
BURMER, GC ;
BRENTNALL, TA ;
STEVENS, AC ;
LEVINE, DS ;
DEAN, PJ ;
KIMMEY, M ;
PERERA, DR ;
RABINOVITCH, PS .
GASTROENTEROLOGY, 1992, 103 (05) :1611-1620
[39]   A review of the S100 proteins in cancer [J].
Salama, I. ;
Malone, P. S. ;
Mihaimeed, F. ;
Jones, J. L. .
EJSO, 2008, 34 (04) :357-364
[40]   Differential protein expression profile in the intestinal epithelium from patients with inflammatory bowel disease [J].
Shkoda, Anna ;
Werner, Tanja ;
Daniel, Hannelore ;
Gunckel, Manuela ;
Rogler, Gerhard ;
Haller, Dirk .
JOURNAL OF PROTEOME RESEARCH, 2007, 6 (03) :1114-1125