Effect of auxins on in vitro rooting of Plumbago zeylanica:: peroxidase activity as a marker for root induction

被引:25
作者
Saxena, C
Samantaray, S
Rout, GR
Das, P [1 ]
机构
[1] Reg Plant Resource Ctr, Plant Tissue Culture Lab, Bhubaneswar 751015, Orissa, India
[2] Reg Plant Resource Ctr, Plant Physiol & Biochem Lab, Bhubaneswar 751015, Orissa, India
关键词
medicinal plant; root initiation; tissue culture;
D O I
10.1023/A:1026519417080
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Induction of rooting in the microshoots of Plumbago zeylanica was achieved on halfstrength basal Murashige and Skoog's medium supplemented with 0.25 mg dm(-3) indole-3-butyric acid. Rooting was totally inhibited when the microshoots were cultured in vitro under continuous light, however, maximum percentage of microshoots rooted when incubated in continuous light for 4 weeks before transfer to the rooting media. Peroxidase activity increased markedly during root induction indicating a key role of peroxidase in rooting of microshoots of Plumbago zeylanica in vitro.
引用
收藏
页码:121 / 124
页数:4
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