Production of a Rat Monoclonal Antibody Against Brg1

被引：11

作者：

Ohkawa, Yasuyuki ^{[2
]}

Harada, Akihito ^{[2
]}

Nakamura, Mako ^{[2
]}

Yoshimura, Saori ^{[1
]}

Tachibana, Taro ^{[1
]}

机构：

[1] Osaka City Univ, Grad Sch Engn, Dept Bioengn, Osaka 5588585, Japan

[2] Kyushu Univ, Fac Med, Dept Epigenet, SSP Stem Cell Unit, Fukuoka 812, Japan

来源：

HYBRIDOMA | 2009年 / 28卷 / 06期

关键词：

MAMMALIAN SWI/SNF COMPLEXES; GENE-EXPRESSION; SNF COMPLEX; ACTIVATION; TRANSCRIPTION; INTERACTS;

D O I：

10.1089/hyb.2009.0041

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Brm-related gene-1 (Brg1) is a catalytic subunit of the SWI/SNF chromatin remodeling enzyme complex that has ATPase activity. This complex facilitates chromatin remodeling for gene expression by utilizing energy for ATP hydrolysis. It is well known that the SWI/SNF chromatin remodeling enzyme complex is essential for cell differentiation, cell cycle regulation, and embryogenesis. Here we report the establishment of a hybridoma cell line for producing an antibody against Brg1 subunit by the rat medial iliac lymph node method. Immunoblot analysis showed that our antibody can specifically recognize Brg1. It was revealed by immunocytochemistry that Brg1 is located in euchromatin of C2C12 myoblast nuclei. These data suggested this antibody is useful for analyzing molecular function of Brg1 protein in cells.

引用

页码：463 / 466

页数：4

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