The Single-molecule long-read sequencing of Scylla paramamosain

被引:23
作者
Wan, Haifu [1 ]
Jia, Xiwei [1 ]
Zou, Pengfei [1 ]
Zhang, Ziping [2 ]
Wang, Yilei [1 ]
机构
[1] Jimei Univ, Fisheries Coll, Minist Agr & Rural Affairs, Key Lab Hlth Mariculture East China Sea, Xiamen 361021, Fujian, Peoples R China
[2] Fujian Agr & Forestry Univ, Coll Anim Sci, Fuzhou 350002, Fujian, Peoples R China
基金
国家重点研发计划;
关键词
CHINESE MITTEN CRAB; BLACKBACK LAND CRAB; DIFFERENTIALLY EXPRESSED GENES; GLAND Y-ORGAN; ERIOCHEIR-SINENSIS; TRANSCRIPTOME ANALYSIS; MOLTING GLAND; RNA-SEQ; GECARCINUS-LATERALIS; HEPATOPANCREAS;
D O I
10.1038/s41598-019-48824-8
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Scylla paramamosain is an important aquaculture crab, which has great economical and nutritional value. To the best of our knowledge, few full-length crab transcriptomes are available. In this study, a library composed of 12 different tissues including gill, hepatopancreas, muscle, cerebral ganglion, eyestalk, thoracic ganglia, intestine, heart, testis, ovary, sperm reservoir, and hemocyte was constructed and sequenced using Pacific Biosciences single-molecule real-time (SMRT) long-read sequencing technology. A total of 284803 full-length non-chimeric reads were obtained, from which 79005 high-quality unique transcripts were obtained after error correction and sequence clustering and redundant. Additionally, a total of 52544 transcripts were annotated against protein database (NCBI nonredundant, Swiss-Prot, KOG, and KEGG database). A total of 23644 long non-coding RNAs (lncRNAs) and 131561 simple sequence repeats (SSRs) were identified. Meanwhile, the isoforms of many genes were also identified in this study. Our study provides a rich set of full-length cDNA sequences for S. paramamosain, which will greatly facilitate S. paramamosain research.
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页数:11
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