The performance of aerobic granular sludge for simulated swine wastewater treatment and the removal mechanism of tetracycline

In this study, aerobic granular sludge (AGS) cultivated in a sequencing batch reactor (SBR) was employed to investigate its ability on the decontamination of tetracycline (TC) from swine wastewater (SWW). The removal mechanism of TC by AGS was studied. Results showed that the AGS process could effectively remove chemical oxygen demand (COD), ammonium nitrogen (NH+ (4)-N), total phosphorus (TP), and TC during operation. The removal of TC by AGS was mainly due to adsorption and biodegradation, and the contribution rate of biodegradation increased after AGS adaptation to TC. Twenty-two by-products were detected during biodegradation of TC, and accordingly the degradation pathway of TC was speculated. Compared to the control reactor, the microbe diversity in different levels of classification was richer in the TC fed reactor according to the LefSe analysis. The results revealed that enzymes that participated in the metabolic pathway of microbial biodegradation of polycyclic aromatic compounds were enriched and may have played a key role in the biodegradation of TC.