Dentin micro-architecture using harmonic generation microscopy

被引:24
作者
Elbaum, R.
Tal, E.
Perets, A. I.
Oron, D.
Ziskind, D.
Silberberg, Y.
Wagner, H. D. [1 ]
机构
[1] Weizmann Inst Sci, Dept Mat & Interfaces, IL-76100 Rehovot, Israel
[2] Weizmann Inst Sci, Phys Complex Syst, IL-76100 Rehovot, Israel
[3] Hebrew Univ Jerusalem, Hadassah Sch Dent Med, Dept Prosthodont, IL-91120 Jerusalem, Israel
关键词
dentin; harmonic generation microscopy; tubules; collagen fibrils; three-dimensional imaging;
D O I
10.1016/j.jdent.2006.07.007
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objectives: We present a novel way to create high-resolution three-dimensional images of tooth dentin by harmonic generation scanning laser microscopy. Methods: The images were taken using a pulsed infrared laser. Three-dimensional reconstruction enables the visualization of individual tubules and the collagen fibrils mesh around them with an optical resolution of similar to 1 mu m. Results: The images show micro-morphological details of the dentinal tubules as well as the collagen fibrils at a depth of up to about 200 mu m. The data show that while collagen fibrils are organized in planes perpendicular to the tubules, close to the dentin enamel junction they lie also along the long axis of the tubules. Conclusions: The unique 3D information opens the opportunity to study the collagen fibril arrangement in relation to the tubule orientation within the dentin matrix, and may be applied to study the. micro-morphology of normal versus altered dentin. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:150 / 155
页数:6
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