Production and characterization of new families of polyglucuronic acids from TEMPO-NaOCl oxidation of curdlan

Curdlan from Agrobacterium sp. was oxidized using 2,2,6,6,-teti-amethylpiperidine-l-oxyl radical (TEMPO)-NaBr-NaCIO systems at pH 11. The effects ofoxiclation conditions on degrees of oxidation polymerization ofthe products obtained were studied using SEC-MALLS, NMR and IR analyses. Different families of water-soluble beta-(1,3)-polyglucuronic and fi-(1,3)-polyglucoglucuronic acid sodium salts were quantitatively generated with a yield of 80% and without significant loss of their molecular weights. Given that beta-(1,3)-polyglucuronic acids prepared from the regioselective oxidation of curdlan by TEMPO-NaBr-NaClO systems regularly consist of the glucuronic acid repeating unit; they may open biotechnological fields for the utilizations of water soluble forms ofcurdlan. (C) 2009 Elsevier B.V. All rights reserved.