Antisense expression of Gossypium hirsutum UDP-glucuronate decarboxylase in Arabidopsis leads to changes in cell wall components

被引：3

作者：

Zhang, D. M. ^{[1
]}

Pan, Y. X. ^{[2
]}

Zhang, Y. ^{[1
]}

Li, Z. K. ^{[1
]}

Wu, L. Q. ^{[1
]}

Liu, H. W. ^{[1
]}

Zhang, G. Y. ^{[1
]}

Wang, X. F. ^{[1
]}

Ma, Z. Y. ^{[1
]}

机构：

[1] Agr Univ Hebei, Key Lab Crop Germplasm Resources Hebei, Educ Minist, North China Key Lab Crop Germplasm Resources, Baoding, Peoples R China

[2] North China Univ Sci & Technol, Tangshan, Peoples R China

来源：

GENETICS AND MOLECULAR RESEARCH | 2016年 / 15卷 / 01期

关键词：

Gossypium hirsutum; UDP-glucuronate decarboxylase; Transgenic Arabidopsis; Down-regulation; Cell wall changes; ACID DECARBOXYLASE; XYLOSE SYNTHASE; COTTON FIBER; DOWN-REGULATION; BIOSYNTHESIS; CLONING; OLIGOSACCHARIDES; POLYSACCHARIDES; ELONGATION; INITIATION;

D O I：

10.4238/gmr.15017409

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

UDP-glucuronate decarboxylase (UDP-xylose synthase; UXS, EC 4.1.1.35) is an essential enzyme of the non-cellulosic polysaccharide biosynthetic pathway. In the present study, using transient expression of fluorescently labeled Gossypium hirsutum UXS (GhUXS3) protein in onion epidermal cells, we observed that this protein was distributed in the cytoplasm. The GhUXS3 cDNA of cotton was expressed in an antisense orientation in Arabidopsis thaliana by Agrobacterium tumefaciens-mediated transformation. Homozygous plants showing down-regulation of UXS were analyzed with northern blots. Compared to the untransformed control, transgenic plant showed shorter roots, earlier blossom formation, and delayed senescence. Biochemical analysis indicated that levels of rhamnose, mannose, galactose, glucose, xylose, and cellulose were reduced in some of the down-regulated antisense plants. These results suggest that GhUXS3 regulates the conversion of non-cellulosic polysaccharides and modulates their composition in plant cell walls. We also discuss a possible cellular function for GhUXS in determining the quality of cotton fibers.

引用

页数：12

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