Role of bacteriophage T4 baseplate in regulating assembly and infection

被引：56

作者：

Yap, Moh Lan ^{[1
]}

Klose, Thomas ^{[1
]}

Arisaka, Fumio ^{[2
]}

Speir, Jeffrey A. ^{[3
]}

Veesler, David ^{[3
,4
]}

Fokine, Andrei ^{[1
]}

Rossmann, Michael G. ^{[1
]}

机构：

[1] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA

[2] Nihon Univ, Coll Bioresource Sci, Fujisawa, Kanagawa 2520880, Japan

[3] Scripps Res Inst, Natl Resource Automated Mol Microscopy, La Jolla, CA 92037 USA

[4] Univ Washington, Dept Biochem, Seattle, WA 98195 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2016年 / 113卷 / 10期

关键词：

bacteriophage T4; cryo-EM reconstruction; baseplate assembly; conformational changes; near-atomic resolution; VI SECRETION SYSTEM; GENETIC-CONTROL; IN-VIVO; TAIL; MORPHOGENESIS; MICROSCOPY; RESOLUTION; DOMAIN;

D O I：

10.1073/pnas.1601654113

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Bacteriophage T4 consists of a head for protecting its genome and a sheathed tail for inserting its genome into a host. The tail terminates with a multiprotein baseplate that changes its conformation from a "high-energy" dome-shaped to a "low-energy" star-shaped structure during infection. Although these two structures represent different minima in the total energy landscape of the baseplate assembly, as the dome-shaped structure readily changes to the star-shaped structure when the virus infects a host bacterium, the dome-shaped structure must have more energy than the star-shaped structure. Here we describe the electron microscopy structure of a 3.3-MDa in vitro-assembled star-shaped baseplate with a resolution of 3.8 angstrom. This structure, together with other genetic and structural data, shows why the high-energy baseplate is formed in the presence of the central hub and how the baseplate changes to the low-energy structure, via two steps during infection. Thus, the presence of the central hub is required to initiate the assembly of metastable, high-energy structures. If the high-energy structure is formed and stabilized faster than the low-energy structure, there will be insufficient components to assemble the low-energy structure.

引用

页码：2654 / 2659

页数：6

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