Specificity of anti-human CD95 (APO-1/Fas) antibodies

被引：6

作者：

Schmitz, I ^{[1
]}

Krueger, A ^{[1
]}

Baumann, S ^{[1
]}

Kirchhoff, S ^{[1
]}

Krammer, PH ^{[1
]}

机构：

[1] German Canc Res Ctr, Tumor Immunol Programme, Div Immunogenet G0300, D-69120 Heidelberg, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 297卷 / 03期

关键词：

apoptosis; CD95/AP0-l/Fas; antibodies; western blot;

D O I：

10.1016/S0006-291X(02)02242-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The CD95 (APO-1/Fas) death receptor plays an important role in many physiological and pathophysiological systems. Thus, the CD95 system contributes to activation-induced cell death. Therefore, reliable antibodies recognizing human CD95 are of great interest. Detection of CD95 expression often relies on antibodies, e.g., suitable for Western blotting. To detect CD95, we compared the specificity of nine different anti-human CD95 antibodies recognizing different epitopes by using postnuclear supernatants of four different cell lines. Only two of the antibodies tested, both directed against intracellular epitopes of human CD95, detected endogenous human CD95 by Western blotting. Therefore, we conclude that results obtained with other anti-CD95 antibodies should be treated with caution. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：459 / 462

页数：4

共 20 条

[1] BUILDING BRIDGES - DISULFIDE BOND FORMATION IN THE CELL [J].