Proteolysis of ankyrin and Na+/K+-ATPase in postmortem rat brain: Is calpain involved?

被引：7
作者：
Harada, K
Sorimachi, Y
Yoshida, K
机构：
[1] YAMAGUCHI UNIV,SCH MED,DEPT LEGAL MED,YAMAGUCHI 755,JAPAN
[2] KYOTO PREFECTURAL UNIV MED,DEPT LEGAL MED,KYOTO 602,JAPAN
来源：
FORENSIC SCIENCE INTERNATIONAL | 1997年 / 86卷 / 1-2期
关键词：
postmortem; ankyrin; cytoskeleton; calpain; Na+/K+-ATPase;
D O I：
10.1016/S0379-0738(97)02120-8
中图分类号：
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号：
0301 ; 10 ;
摘要：
Ankyrin links the fodrin-based cytoskeleton to membrane proteins such as Na+/K+-ATPase, thereby maintaining cellular integrity. Immunoblotting by antibody raised against erythrocyte ankyrin demonstrated the proteolysis of ankyrin, which was highly correlated with postmortem interval (0-24 h). Proteolysis in the postmortem brain generated the 160-kDa fragment with an identical size as the fragment formed after in vitro proteolysis by calpain. Although mu M Ca2+ induced the proteolysis in the homogenate, the presence of mu-calpain was not demonstrated by immunoblotting using the antibody that reacts with large subunits both of mu- and m-calpains. Na+/K+-ATPase was also proteolyzed in the postmortem brain. (C) 1997 Elsevier Science Ireland Ltd.
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收藏
页码：77 / 85
页数：9
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