Prevalence of the Crayfish Plague Pathogen Aphanomyces astaci in Invasive American Crayfishes in the Czech Republic

被引:72
作者
Kozubikova, Eva [1 ,2 ]
Filipova, L. [1 ]
Kozak, P. [2 ]
Duris, Z. [3 ]
Martin, M. P. [4 ]
Dieguez-Uribeondo, J. [4 ]
Oidtmann, B. [5 ]
Petrusek, A. [1 ,2 ]
机构
[1] Charles Univ Prague, Fac Sci, Dept Ecol, CZ-12844 Prague 2, Czech Republic
[2] Univ S Bohemia Ceske Budejovice, Res Inst Fish Culture & Hydrobiol, CZ-38925 Vodnany, Czech Republic
[3] Univ Ostrava, Dept Biol & Ecol, CZ-71000 Ostrava, Czech Republic
[4] Real Jardin Bot CSIC, Dept Micol, Madrid 28014, Spain
[5] Cefas Weymouth Lab, Weymouth DT4 8UB, Dorset, England
关键词
Aphanomyces astaci; crayfish plague; distribution; fungal pathogen; infection ratio; invasive species; Orconectes limosus; Pacifastacus leniusculus; FRESH-WATER CRAYFISH; SIGNAL CRAYFISH; PACIFASTACUS-LENIUSCULUS; ORCONECTES-LIMOSUS; FUNGUS; CLASSIFICATION; SAMPLES; SPREAD; PCR; DNA;
D O I
10.1111/j.1523-1739.2009.01240.x
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
In Central Europe invasive North American crayfishes are carriers of the oomycete Aphanomyces astaci, which causes crayfish plague. This lethal disease currently represents one of the major threats to native European crayfishes. We used molecular methods-species-specific amplification and sequencing of the pathogen DNA-to investigate the prevalence of individuals latently infected with A. astaci in 28 populations of two invasive American crayfish species (6 of the signal crayfish [Pacifastacus leniusculus] and 22 of the spiny-cheek crayfish [Orconectes limosus]) in the Czech Republic. The pathogen occurred in 17 investigated populations. We recorded a high variation in positive reactions, ranging from 0% to 100%, in populations of O. limosus. In P. leniusculus, however, only one individual out of 124 tested positive for the pathogen. There was a clear relationship between the water body type and pathogen prevalence in O. limosus. Infection ratios in isolated standing waters were usually low, whereas in running waters, pathogen prevalence often exceeded 50%. Other evaluated characteristics of potential plague pathogen carriers (size, sex, and the presence of melanized spots in the cuticle) seemed to be unrelated to infection. Our data suggest that in contrast to other European countries, O. limosus seems to be the primary reservoir of crayfish plague in the Czech Republic. Although all populations of alien American crayfishes may be potential sources of infections and should be managed as such, knowledge on the prevalence of the plague pathogen at various localities may allow managers to focus conservation efforts on the most directly endangered populations of native crayfishes.
引用
收藏
页码:1204 / 1213
页数:10
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