Different role of platelet glycoprotein GP Ia/IIa in platelet contact and activation induced by type I and type III collagens

被引：13
作者：
Monnet, E
Sizaret, PY
Arbeille, B
Fauvel-Lafève, F
机构：
[1] Univ Paris 07, Inst Hematol, Hop St Louis, INSERM,U353, F-75475 Paris 10, France
[2] Hop Bretonneau, Unite Microscopie Elect, UER Med, F-37000 Tours, France
来源：
THROMBOSIS RESEARCH | 2000年 / 98卷 / 05期
关键词：
platelet adhesion; collagen; GP Ia/IIa; platelet activation;
D O I：
10.1016/S0049-3848(00)00199-7
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The role of glycoprotein Ia/IIa was studied during platelet contact and aggregation induced by type I and type III collagen. The anti-glycoprotein Ia/IIa (6F1) antibody inhibited type I collagen-induced aggregation but did not inhibit the first contact between platelets and collagen. In contrast, it was without effect either on type III collagen-induced contact or platelet interaction with the subendothelium in a static assay. Platelet aggregation induced by type III collagen was only slightly slowed down by 6F1 but pp72 spleen tyrosine kinase phosphorylation was not modified even at concentrations of 6F1 that completely blocked platelet activation induced by type I collagen. Our results indicate that glycoprotein Ia/IIa is not a primary binding site for type I or type III collagen on the platelet membrane. This receptor is more specifically involved in type I collagen-induced platelet spreading and aggregation. (C) 2000 Elsevier Science Ltd. All rights reserved.
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收藏
页码：423 / 433
页数：11
相关论文
共 40 条
[1]  
Asazuma N, 1996, THROMB HAEMOSTASIS, V75, P648
[2]   Collagen-like peptide stimulates tyrosine phosphorylation of syk and phospholipase C gamma 2 in platelets independent of the integrin alpha(2)beta(1) [J].
Asselin, J ;
Gibbins, JM ;
Achison, M ;
Lee, YH ;
Morton, LF ;
Farndale, RW ;
Barnes, MJ ;
Watson, SP .
BLOOD, 1997, 89 (04) :1235-1242
[3]   ROLE OF BLOOD-FLOW IN PLATELET ADHESION, FIBRIN DEPOSITION, AND FORMATION OF MURAL THROMBI [J].
BAUMGARTNER, HR .
MICROVASCULAR RESEARCH, 1973, 5 (02) :167-179
[4]   PLATELET-ADHESION, RELEASE AND AGGREGATION IN FLOWING BLOOD - EFFECTS OF SURFACE PROPERTIES AND PLATELET-FUNCTION [J].
BAUMGARTNER, HR ;
MUGGLI, R ;
TSCHOPP, TB ;
TURITTO, VT .
THROMBOSIS AND HAEMOSTASIS, 1976, 35 (01) :124-138
[5]   A COLORIMETRIC METHOD FOR THE MEASUREMENT OF PLATELET-ADHESION IN MICROTITER PLATES [J].
BELLAVITE, P ;
ANDRIOLI, G ;
GUZZO, P ;
ARIGLIANO, P ;
CHIRUMBOLO, S ;
MANZATO, F ;
SANTONASTASO, C .
ANALYTICAL BIOCHEMISTRY, 1994, 216 (02) :444-450
[6]  
BONEU B, 1987, SANG VAISSEAUX, P141
[7]   A synthetic peptide derived from the sequence of a type I collagen receptor inhibits type collagen-mediated platelet aggregation [J].
Chiang, TM ;
Kang, AH .
JOURNAL OF CLINICAL INVESTIGATION, 1997, 100 (08) :2079-2084
[8]   Cloning, characterization, and functional studies of a nonintegrin platelet receptor for type I collagen [J].
Chiang, TM ;
Rinaldy, A ;
Kang, AH .
JOURNAL OF CLINICAL INVESTIGATION, 1997, 100 (03) :514-521
[9]   COLLAGEN-PLATELET INTERACTION - SEPARATE RECEPTOR-SITES FOR TYPE-I AND TYPE-III COLLAGEN [J].
CHIANG, TM ;
SEYER, JM ;
KANG, AH .
THROMBOSIS RESEARCH, 1993, 71 (06) :443-456
[10]  
CHIANG TM, 1987, J IMMUNOL, V139, P887
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