Occurrence, prevalence and genetic environment of CTX-M β-lactamases in Enterobacteriaceae from Indian hospitals

被引:101
作者
Ensor, V. M.
Shahid, M.
Evans, J. T.
Hawkey, P. M.
机构
[1] Univ Birmingham, Inst Biomed Res, Antimicrobial Agents Res Grp, Birmingham B15 2TT, W Midlands, England
[2] Heart England NHS Fdn Trust, W Midlands Hlth Protect Agcy, Birmingham B9 5SS, W Midlands, England
[3] Aligarh Muslim Univ, Jawaharlal Nehru Med Coll & Hosp, Dept Microbiol, Aligarh 202002, Uttar Pradesh, India
基金
英国生物技术与生命科学研究理事会;
关键词
ESBLs; IS26; CTX-M-15; India;
D O I
10.1093/jac/dkl422
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Objectives: To determine occurrence, prevalence and CTX-M genotypes produced by Enterobacteriaceae from clinical samples from three geographically distant Indian hospitals and to detect linkage of IS26 with bla(CTX-M) and map its precise insertion position. Methods: A total of 130, non-duplicate Escherichia coli and Klebsiella pneumoniae resistant to a third-generation cephalosporin (3GC) from three Indian centres were screened for extended-spectrum beta-lactamase (ESBL) production using phenotypic detection methods. All isolates were screened for bla(CTX-M) using multiplex PCR. Precise CTX-M genotype was identified using reverse-line hybridization. All CTX-M-producing isolates were screened for linkage of IS26 with bla(CTX-M). DNA sequencing was used to map the exact insertion position of this mobile element. Results: Ninety-five of 130 3GC-resistant (73%) (73% of total E. coli, 72% of total K. pneumoniae) isolates were found to carry bla(CTX-M-15). No other CTX-M genotype was detected. IS26 linkage with bla(CTX-M-15) was detected in 31% of isolates carrying bla(CTX-M-15). DNA sequencing revealed variable insertion of this mobile element within tnpA of ISEcp1. RAPD-PCR typing demonstrated great diversity in isolates carrying bla(CTX-M-15); no predominant clone was identified. Conclusions: In contrast with other studies where greater diversity exists, CTX-M-15 was the only CTX-M ESBL produced in this Indian collection of unrelated E. coli and K. pneumoniae. This is the first systematic survey report from India detecting CTX-M-type beta-lactamases This is also the first report indicating such high mobility/diversity of insertion of IS26 in close association with bla(CTX-M) in a single bacterial collection.
引用
收藏
页码:1260 / 1263
页数:4
相关论文
共 16 条
[1]   Growing group of extended-spectrum β-lactamases:: The CTX-M enzymes [J].
Bonnet, R .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (01) :1-14
[2]   Detection of extended-spectrum β-lactamases in klebsiellae with the oxoid combination disk method [J].
Carter, MW ;
Oakton, KJ ;
Warner, M ;
Livermore, DM .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (11) :4228-4232
[3]  
Datta P, 2004, JPN J INFECT DIS, V57, P146
[4]   DNA sequence analysis of the genetic environment of various blaCTX-M genes [J].
Eckert, C ;
Gautier, V ;
Arlet, G .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2006, 57 (01) :14-23
[5]  
ENSOR V, 2006, CLIN MICROBIOL IN S4, V12
[6]   Evolutionary mapping of the SHV β-lactamase and evidence for two separate IS26-dependent blaSHV mobilization events from the Klebsiella pneumoniae chromosome [J].
Ford, PJ ;
Avison, MB .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2004, 54 (01) :69-75
[7]   Multidrug resistance in gram-negative bacteria that produce extended-spectrum β-lactamases (ESBLs) [J].
Giamarellou, H .
CLINICAL MICROBIOLOGY AND INFECTION, 2005, 11 :1-16
[8]   Phenotypic and genotypic detection of ESBL mediated cephalosporin resistance in Klebsiella pneumoniae:: Emergence of high resistance against cefepime, the fourth generation cephalosporin [J].
Grover, S. S. ;
Sharma, Meenakshi ;
Chattopadhya, D. ;
Kapoor, Hema ;
Pasha, S. T. ;
Singh, Gajendra .
JOURNAL OF INFECTION, 2006, 53 (04) :279-288
[9]   Plasmid-mediated extended-spectrum β-lactamase (CTX-M-3 like) from India and gene association with insertion sequence ISEcp1 [J].
Karim, A ;
Poirel, L ;
Nagarajan, S ;
Nordmann, P .
FEMS MICROBIOLOGY LETTERS, 2001, 201 (02) :237-241
[10]   The β-lactamase threat in Enterobacteriaceae, Pseudomonas and Acinetobacter [J].
Livermore, David M. ;
Woodford, Neil .
TRENDS IN MICROBIOLOGY, 2006, 14 (09) :413-420