Two-photon fluorescence imaging of microspheres embedded in turbid media

被引：7
|
作者：
Gu, M [1 ]
Schilders, SP [1 ]
Gan, X [1 ]
机构：
[1] Victoria Univ, Sch Commun & Informat, Optoelect Imaging Grp, Melbourne, Vic 8001, Australia
来源：
JOURNAL OF MODERN OPTICS | 2000年 / 47卷 / 06期
基金：
澳大利亚研究理事会;
关键词：
D O I：
10.1080/09500340008233398
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Fluorescent microspheres of diameter 10 mu m embedded in a turbid medium consisting of polystyrene beads suspended in water are imaged under two-photon and single-photon excitation. A comparison of two-photon and single-photon fluorescence images shows that multiple scattering leads to a dominant limiting factor of the signal-to-noise ratio in the former case, while it results in a dominant limiting factor of resolution in the latter case. These results are qualtitatively consistent with the predication by the Monte-Carlo simulation based on Mie scattering theory.
引用
收藏
页码：959 / 965
页数：7
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