Application of the Droplet Digital Polymerase Chain Reaction (ddPCR) Platform for Detection and Quantification of Vertebrate Host DNA in Engorged Mosquitoes

被引：1

作者：

Rice, L. M. ^{[1
,2
]}

Robb, L. L. ^{[1
]}

Hartman, D. A. ^{[1
]}

Anderson, J. R. ^{[1
]}

Kading, R. C. ^{[1
]}

机构：

[1] Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA

[2] Univ Colorado, Sch Med, Dept Pathol, Denver, CO 80262 USA

来源：

JOURNAL OF MEDICAL ENTOMOLOGY | 2019年 / 56卷 / 04期

基金：

美国食品与农业研究所;

关键词：

diagnostics; feeding behavior; mosquito-borne disease; survey; detection; BLOOD MEALS; CULICIDAE; DIPTERA; PCR; IDENTIFICATION; VARIANTS;

D O I：

10.1093/jme/tjz016

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Hematophagous arthropod bloodmeal identification has remained a challenge in the field of vector biology, but these studies are important to understand blood feeding patterns of arthropods, spatial, and temporal patterns in arbovirus transmission cycles, and risk of human and veterinary disease. We investigated the use of an existing vertebrate primer set for use on the droplet digital polymerase chain reaction (ddPCR) platform, to explore the use of this technology in the identification and quantification of vertebrate DNA in mosquito blood meals. Host DNA was detectable 48-h post-engorgement in some mosquitoes by ddPCR, compared with 24-h post-engorgement using traditional PCR. The capability of ddPCR for absolute quantification of template DNA offers unique potential applications of this new technology to field studies on the ecology of vector-borne diseases, but currently with limited scope.

引用

收藏

页码：1150 / 1153

页数：4

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