Eukaryotic DNA replication control: Lock and load, then fire

被引：171

作者：

Remus, Dirk ^{[1
]}

Diffley, John F. X. ^{[1
]}

机构：

[1] Canc Res UK London Res Inst, Clare Hall Labs, S Mimms EN6 3LD, Herts, England

来源：

CURRENT OPINION IN CELL BIOLOGY | 2009年 / 21卷 / 06期

关键词：

ESCHERICHIA-COLI; GINS COMPLEX; CRYSTAL-STRUCTURE; DORMANT ORIGINS; MCM PROTEINS; E; COLI; S-PHASE; INITIATION; ORC; PHOSPHORYLATION;

D O I：

10.1016/j.ceb.2009.08.002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The initiation of chromosomal DNA replication involves initiator proteins that recruit and load hexameric DNA helicases at replication origins. This helicase loading step is tightly regulated in bacteria and eukaryotes. In contrast to the situation in bacteria, the eukaryotic helicase is loaded in an inactive form. This extra 'lock and load' mechanism in eukaryotes allows regulation of a second step, helicase activation. The temporal separation of helicase loading and activation is crucial for the coordination of DNA replication with cell growth and extracellular signals, the prevention of rereplication and the control of origin activity in response to replication stress. Initiator proteins in bacteria and eukaryotes are structurally homologous; yet the replicative helicases they load are unrelated. Understanding how these helicases are loaded and how they act during unwinding may have important implications for understanding how DNA replication is regulated in different domains of life.

引用

页码：771 / 777

页数：7

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