MicroRNA-221 Promotes Cell Proliferation and Inhibits Apoptosis in Osteosarcoma Cells by Directly Targeting FBXW11 and Regulating Wnt Signaling
被引:18
|
作者:
Zhang, Qingzhu
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机构:
Chengde Med Univ, Dept Orthoped, Affiliated Hosp, Chengde, Peoples R ChinaChengde Med Univ, Dept Orthoped, Affiliated Hosp, Chengde, Peoples R China
Zhang, Qingzhu
[1
]
Yin, Xuelian
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机构:
Chengde Med Univ, Dept Stomatol, Affiliated Hosp, Chengde, Peoples R ChinaChengde Med Univ, Dept Orthoped, Affiliated Hosp, Chengde, Peoples R China
Yin, Xuelian
[2
]
Zhang, Yi
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机构:
Chengde Med Univ, Dept Orthoped Trauma, Affiliated Hosp, Chengde 067000, Hebei, Peoples R ChinaChengde Med Univ, Dept Orthoped, Affiliated Hosp, Chengde, Peoples R China
Zhang, Yi
[3
]
机构:
[1] Chengde Med Univ, Dept Orthoped, Affiliated Hosp, Chengde, Peoples R China
[2] Chengde Med Univ, Dept Stomatol, Affiliated Hosp, Chengde, Peoples R China
[3] Chengde Med Univ, Dept Orthoped Trauma, Affiliated Hosp, Chengde 067000, Hebei, Peoples R China
microRNAs;
miR-221;
FBXW11;
Osteosarcoma cells;
Cancer progression;
D O I:
10.1016/j.arcmed.2020.10.017
中图分类号:
R-3 [医学研究方法];
R3 [基础医学];
学科分类号:
1001 ;
摘要:
Background and objectives. MicroRNAs play a crucial role in the progression of various cancers, and microRNA-221 (miR-221) has been observed to be significantly overexpressed in osteosarcoma (OS) cells. FBXW11, a vital F-box protein of the ubiquitin-proteasome system, mediates the proliferation and survival of cancer cells by targeting multiple substrates for degradation. FBXW11 inhibits OS growth and metastasis by antagonizing the beta-catenin/Wnt signaling pathway. Therefore, we hypothesized that miR-221 targets FBXW11 to mediate Wnt signaling and promote OS proliferation. Methods. In this study, we demonstrated the increased expression of miR-221 and FBXW11 in OS tissues and cell lines by real-time polymerase chain reaction (RT-PCR). Moreover, to elucidate the regulatory mechanism(s) of miR-221 and FBXW11 in progression, cell viability and apoptosis were analyzed by the MTT assay and flow cytometry, respectively. Results. The results showed that the overexpression of miR-221 in OS cells dramatically promoted cell growth and cell cycle progression, and inhibited apoptosis, whereas miR-221 inhibitors conversely inhibited proliferation and promoted apoptosis in OS cells. The data also showed that FBXW11 directly targeted miR-221 and miR-221 regulated OS cell proliferation and apoptosis by binding to FBXW11. We further confirmed that miR-221 targeted FBXW11 to promote proliferation and inhibit apoptosis in OS cell lines by inhibiting Wnt signaling. Interpretation and conclusions. Overall, our study revealed a functional mechanism for miR-221 in OS. Further studies will elucidate its role in the progression of OS and inhibiting miR-221 may represent a useful treatment strategy. (C) 2021 IMSS. Published by Elsevier Inc.
机构:
Shanghai Jiao Tong Univ, Oncol Dept, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R ChinaShanghai Jiao Tong Univ, Oncol Dept, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R China
Zhang, Jianjun
Sha, Jingjing
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Jin Yang Community Hlth Serv Ctr, Shanghai 200136, Peoples R ChinaShanghai Jiao Tong Univ, Oncol Dept, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R China
Sha, Jingjing
Zhou, Yan
论文数: 0引用数: 0
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机构:
Shanghai Jiao Tong Univ, Oncol Dept, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R ChinaShanghai Jiao Tong Univ, Oncol Dept, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R China
Zhou, Yan
Han, Kun
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Shanghai Jiao Tong Univ, Oncol Dept, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R ChinaShanghai Jiao Tong Univ, Oncol Dept, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R China
Han, Kun
Wang, Yaling
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Shanghai Jiao Tong Univ, Oncol Dept, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R ChinaShanghai Jiao Tong Univ, Oncol Dept, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R China
Wang, Yaling
Su, Yang
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Shanghai Jiao Tong Univ, Oncol Dept, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R ChinaShanghai Jiao Tong Univ, Oncol Dept, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R China
Su, Yang
Yin, Xiuyi
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Shanghai Jiao Tong Univ, Oncol Dept, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R ChinaShanghai Jiao Tong Univ, Oncol Dept, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R China
Yin, Xiuyi
Hu, Haiyan
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Shanghai Jiao Tong Univ, Oncol Dept, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R ChinaShanghai Jiao Tong Univ, Oncol Dept, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R China
Hu, Haiyan
Yao, Yang
论文数: 0引用数: 0
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机构:
Shanghai Jiao Tong Univ, Oncol Dept, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R ChinaShanghai Jiao Tong Univ, Oncol Dept, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R China
机构:
Chinese Acad Med Sci, Inst Hematol, Tianjin, Peoples R China
Chinese Acad Med Sci, Blood Dis Hosp, Tianjin, Peoples R China
Peking Union Med Coll, Tianjin, Peoples R ChinaChinese Acad Med Sci, Inst Hematol, Tianjin, Peoples R China
Wang, Lina
Liao, Jinfeng
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Chinese Acad Med Sci, Inst Hematol, Tianjin, Peoples R China
Chinese Acad Med Sci, Blood Dis Hosp, Tianjin, Peoples R China
Peking Union Med Coll, Tianjin, Peoples R ChinaChinese Acad Med Sci, Inst Hematol, Tianjin, Peoples R China
Liao, Jinfeng
Feng, Wenli
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Chinese Acad Med Sci, Inst Hematol, Tianjin, Peoples R China
Chinese Acad Med Sci, Blood Dis Hosp, Tianjin, Peoples R China
Peking Union Med Coll, Tianjin, Peoples R ChinaChinese Acad Med Sci, Inst Hematol, Tianjin, Peoples R China
Feng, Wenli
Yang, Xiao
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Chinese Acad Med Sci, Inst Hematol, Tianjin, Peoples R China
Chinese Acad Med Sci, Blood Dis Hosp, Tianjin, Peoples R China
Peking Union Med Coll, Tianjin, Peoples R ChinaChinese Acad Med Sci, Inst Hematol, Tianjin, Peoples R China
Yang, Xiao
Chen, Shayan
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Chinese Acad Med Sci, Inst Hematol, Tianjin, Peoples R China
Chinese Acad Med Sci, Blood Dis Hosp, Tianjin, Peoples R China
Peking Union Med Coll, Tianjin, Peoples R ChinaChinese Acad Med Sci, Inst Hematol, Tianjin, Peoples R China
Chen, Shayan
Zheng, Guoguang
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机构:
Peking Union Med Coll, Tianjin, Peoples R China
Chinese Acad Med Sci, Tianjin, Peoples R ChinaChinese Acad Med Sci, Inst Hematol, Tianjin, Peoples R China
机构:
Jilin Univ, Dept Orthoped, Hosp 2, 218 Ziqiang St, Changchun 130000, Jilin, Peoples R ChinaJilin Univ, Dept Orthoped, Hosp 2, 218 Ziqiang St, Changchun 130000, Jilin, Peoples R China
Zhao, Xin
Sun, Shicai
论文数: 0引用数: 0
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机构:
Songyuan Integrated Tradit Chinese & Western Med, Dept Orthoped, Songyuan, Jilin, Peoples R ChinaJilin Univ, Dept Orthoped, Hosp 2, 218 Ziqiang St, Changchun 130000, Jilin, Peoples R China
Sun, Shicai
Xu, Jianting
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Jilin Univ, Hosp 1, Ctr Canc, Changchun, Jilin, Peoples R ChinaJilin Univ, Dept Orthoped, Hosp 2, 218 Ziqiang St, Changchun 130000, Jilin, Peoples R China
Xu, Jianting
Luo, Yang
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Gen Hosp Chinese PLA, Dept Orthopaed, Beijing, Peoples R ChinaJilin Univ, Dept Orthoped, Hosp 2, 218 Ziqiang St, Changchun 130000, Jilin, Peoples R China
机构:
Jiangsu Normal Univ, Sch Life Sci, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 221116, Peoples R ChinaJiangsu Normal Univ, Sch Life Sci, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 221116, Peoples R China
Zhang, Zi-Feng
Wang, Yong-Jian
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Jiangsu Normal Univ, Sch Life Sci, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 221116, Peoples R ChinaJiangsu Normal Univ, Sch Life Sci, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 221116, Peoples R China
Wang, Yong-Jian
Fan, Shao-Hua
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Jiangsu Normal Univ, Sch Life Sci, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 221116, Peoples R ChinaJiangsu Normal Univ, Sch Life Sci, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 221116, Peoples R China
Fan, Shao-Hua
Du, Shi-Xin
论文数: 0引用数: 0
h-index: 0
机构:
Shenzhen Univ, Affiliated Hosp 3, Dept Orthoped, Shenzhen 518002, Peoples R ChinaJiangsu Normal Univ, Sch Life Sci, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 221116, Peoples R China
Du, Shi-Xin
Li, Xue-Dong
论文数: 0引用数: 0
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机构:
Shenzhen Univ, Affiliated Hosp 3, Dept Orthoped, Shenzhen 518002, Peoples R ChinaJiangsu Normal Univ, Sch Life Sci, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 221116, Peoples R China
Li, Xue-Dong
Wu, Dong-Mei
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机构:
Jiangsu Normal Univ, Sch Life Sci, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 221116, Peoples R ChinaJiangsu Normal Univ, Sch Life Sci, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 221116, Peoples R China
Wu, Dong-Mei
Lu, Jun
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机构:
Jiangsu Normal Univ, Sch Life Sci, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 221116, Peoples R ChinaJiangsu Normal Univ, Sch Life Sci, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 221116, Peoples R China
Lu, Jun
Zheng, Yuan-Lin
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h-index: 0
机构:
Jiangsu Normal Univ, Sch Life Sci, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 221116, Peoples R ChinaJiangsu Normal Univ, Sch Life Sci, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 221116, Peoples R China