Magnetic Fe3S4 nanoparticles with peroxidase-like activity, and their use in a photometric enzymatic glucose assay

被引：115
作者：
Ding, Caiping [1 ]
Yan, Yinghan [1 ]
Xiang, Dongshan [2 ]
Zhang, Cuiling [1 ]
Xian, Yuezhong [1 ]
机构：
[1] E China Normal Univ, Dept Chem, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China
[2] Hubei Univ Nationalities, Sch Chem & Environm Engn, Enshi 445000, Peoples R China
来源：
MICROCHIMICA ACTA | 2016年 / 183卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Greigite nanoparticles; Peroxidase mimic; Hydrogen peroxide; Glucose assay; SEM; TEM; COLORIMETRIC DETECTION; CATALYTIC-ACTIVITY; HYDROGEN-PEROXIDE; MIMETICS; OXIDASE; NANOCOMPOSITE; DOTS; H2O2;
D O I：
10.1007/s00604-015-1690-6
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Greigite magnetic nanoparticles (Fe3S4-MNPs) were prepared and reveal a peroxidase-like activity. Kinetic studies revealed a pseudo-enzymatic activity that is much higher than that of other magnetic nanomaterial-based enzyme mimetics. This finding was exploited to design a photometric enzymatic glucose assay based on the formation of H2O2 during enzymatic oxidation of glucose by glucose oxidase, and the formation of a blue product from an enzyme substrate that is catalytically oxidized by H2O2 in the presence of Fe3S4-MNPs. Glucose can be detected in the 2 to 100 mu M concentration range, and the low detection limit is 0.16 mu M. The method was applied to quantify glucose in human serum. In our perception, this enzyme mimetic has a large potential in that it may be used in other oxidase based assays, but also in ELISAs.
引用
收藏
页码：625 / 631
页数：7
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