Antibiotic resistance profile in relation to virulence genes fimH, hlyA and usp of uropathogenic E. coli isolates in Lahore, Pakistan

被引:0
作者
Ahmed, N. [1 ]
Zeshan, B. [1 ]
Naveed, M. [1 ]
Afzal, M. [1 ]
Mohamed, M. [2 ]
机构
[1] Univ Cent Punjab, Fac Life Sci, Lahore, Pakistan
[2] Univ Malaysia Kelantan, Fac Vet Med, Jeli, Kelantan, Malaysia
关键词
URINARY-TRACT-INFECTION; ESCHERICHIA-COLI; PREVALENCE;
D O I
暂无
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Uropathogenic E. coli (UPEC) is the major etiological agent of urinary tract infections. The objectives of this study were to evaluate E. coli isolates from these patients for the phenotypic pattern of antibiotic resistance and to detect the genes responsible for virulence namely fimH, hlyA and usp. A total of 110 E. coli isolates were studied and 30 antibiotics were applied for susceptibility testing. PCR detection of the genes fimH, hlyA and usp was done followed by sequencing and phylogenetic analysis. The results showed that the isolates were highly resistant to cephalaxin (100%) and cephradine (100%) but displayed high sensitivity to amikacin (96.27%), Imipenem (92.63%) and Meropenem (92.63%). The prevalence of fimH, hlyA and usp was 86%, 83% and 16%, respectively. The sequencing showed 99% similarity with previously reported sequences in NCBI GenBank database. The co-existence of multiple drug-resistant bodies and virulent genes has important implications for the treatment of patients with urinary tract infection. This study provides information about treating drug-resistant E. coli and the relationship of virulent genes with phenotypic resistance patterns.
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页码:559 / 568
页数:10
相关论文
共 27 条
[1]  
Ahmed HF, 2018, TROP BIOMED, V35, P321
[2]  
Ahmed N., 2018, INT J APPL BIOL FORE, V2, P188
[3]   Intracellular bacterial communities of uropathogenic Escherichia coli in urinary tract pathogenesis [J].
Anderson, GG ;
Dodson, KW ;
Hooton, TM ;
Hultgren, SJ .
TRENDS IN MICROBIOLOGY, 2004, 12 (09) :424-430
[4]   Incidence and risk of co-transmission of plasmid-mediated quinolone resistance and extended-spectrum β-lactamase genes in fluoroquinolone-resistant uropathogenic Escherichia coli: a first study from Kolkata, India [J].
Basu, Shreya ;
Mukherjee, Mandira .
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, 2018, 14 :217-223
[5]   Molecular epidemiology of 3 putative virulence genes for Escherichia coli urinary tract infection -: usp, iha, and iroNE-coli [J].
Bauer, RJ ;
Zhang, LX ;
Foxman, B ;
Siitonen, A ;
Jantunen, ME ;
Saxen, H ;
Marrs, CF .
JOURNAL OF INFECTIOUS DISEASES, 2002, 185 (10) :1521-1524
[6]   Detection of virulence factors in α-haemolytic Escherichia coli strains isolated from various clinical materials [J].
Birosová, E ;
Siegfried, L ;
Kmet'ová, M ;
Makara, A ;
Ostró, A ;
Gresová, A ;
Urdzík, P ;
Liptáková, A ;
Molokácová, M ;
Bártl, R ;
Valansky, L .
CLINICAL MICROBIOLOGY AND INFECTION, 2004, 10 (06) :569-573
[7]   Infection strategies of enteric pathogenic Escherichia coli [J].
Clements, Abigail ;
Young, Joanna C. ;
Constantinou, Nicholas ;
Frankel, Gad .
GUT MICROBES, 2012, 3 (02) :71-87
[8]   Virulence Characteristics and Antibiotic Resistance Patterns among Various Phylogenetic Groups of Uropathogenic Escherichia coli Isolates [J].
Derakhshandeh, Abdollah ;
Firouzi, Roya ;
Motamedifar, Mohammad ;
Arabshahi, Sina ;
Novinrooz, Aytak ;
Boroojeni, Azar Motamedi ;
Bahadori, Maryam ;
Heidari, Susan .
JAPANESE JOURNAL OF INFECTIOUS DISEASES, 2015, 68 (05) :428-431
[9]   Integrin-mediated host cell invasion by type 1-piliated uropathogenic Escherichia coli [J].
Eto, Danelle S. ;
Jones, Tiffani A. ;
Sundsbak, Jamie L. ;
Mulvey, Matthew A. .
PLOS PATHOGENS, 2007, 3 (07) :949-961
[10]   Characterization of uropathogenic Escherichia coli strains obtained from urology outpatient clinic of Ege Medical Faculty in Izmir [J].
Giray, Betul ;
Ucar, Fusun Bahriye ;
Aydemir, Sabire Sohret .
TURKISH JOURNAL OF MEDICAL SCIENCES, 2012, 42 :1328-1337