Effects of forespore-specific overexpression of apurinic/apyrimidinic endonuclease Nfo on the DNA-damage resistance properties of Bacillus subtilis spores

被引：9

作者：

Barraza-Salas, Marcelo ^{[1
]}

Ibarra-Rodriguez, Juan R. ^{[1
]}

Mellado, Silvia J. ^{[1
]}

Salas-Pacheco, Jose M. ^{[1
]}

Setlow, Peter ^{[2
]}

Pedraza-Reyes, Mario ^{[1
]}

机构：

[1] Univ Guanajuato, Div Nat & Exact Sci, Dept Biol, Guanajuato 36050, Mexico

[2] Univ Connecticut, Ctr Hlth, Dept Mol Microbial & Struct Biol, Farmington, CT USA

来源：

FEMS MICROBIOLOGY LETTERS | 2010年 / 302卷 / 02期

关键词：

Bacillus subtilis; spores; spore resistance; DNA repair; DNA protection; PROTEIN; REPAIR; HEAT; YQFS;

D O I：

10.1111/j.1574-6968.2009.01845.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The effects of overexpression of the apurinic/apyrimidinic DNA endonuclease Nfo on wet and dry heat and UV-C (254 nm) resistance of Bacillus subtilis spores with or without alpha/beta-type small, acid-soluble spore proteins (SASP) were determined. Results revealed that overexpression of Nfo >= 50-fold in spores increased the wet heat resistance of exoA nfo B. subtilis spores (termed alpha-beta-) that lack most alpha/beta-type SASP, but had no effect on these spores' UV-C resistance. Nfo overexpression also increased these spores' dry heat resistance, and to levels slightly greater than that of wild-type spores. These results are consistent: (1) with wet and dry heat (but not UV-C) generating abasic sites in alpha-beta- spore DNA; (2) with dry heat generating some of these lesions in spores that retain alpha/beta-type SASP; and (3) indicate that Nfo can repair these abasic lesions following spore germination.

引用

页码：159 / 165

页数：7

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