Mechanism and In Vivo Evaluation: Photodynamic Antibacterial Chemotherapy of Lysine-Porphyrin Conjugate

被引：50

作者：

Xu, Zengping ^{[1
]}

Gao, Yuxiang ^{[2
]}

Meng, Shuai ^{[1
,3
]}

Yang, Baochen ^{[1
]}

Pang, Liyun ^{[1
]}

Wang, Chen ^{[3
]}

Liu, Tianjun ^{[1
]}

机构：

[1] Chinese Acad Med Sci, Peking Union Med Coll, Inst Biomed Engn, Tianjin Key Lab Biomed Mat, Tianjin, Peoples R China

[2] Nankai Univ, Dept Mat Chem, Tianjin 300071, Peoples R China

[3] Tianjin Med Univ, Canc Inst & Hosp, Tianjin, Peoples R China

来源：

FRONTIERS IN MICROBIOLOGY | 2016年 / 7卷

基金：

中国国家自然科学基金;

关键词：

lysine-porphyrin conjugate; photodynamic antimicrobial chemotherapy; antibiotic resistance; photosensitizer; mixed bacterial-infected wounds; GRAM-NEGATIVE BACTERIA; STAPHYLOCOCCUS-AUREUS; METHYLENE-BLUE; ANTIMICROBIAL CHEMOTHERAPY; METHICILLIN-RESISTANT; INACTIVATION; PHTHALOCYANINES; INFECTIONS; THERAPY; LIGHT;

D O I：

10.3389/fmicb.2016.00242

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Lysine-porphyrin conjugate 4i has potent photosensitive antibacterial effect on clinical isolated bacterial strains such as Methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coil, and Pseudomonas aeruginosa. The mechanism of photodynamic antibacterial chemotherapy of 4i (4i-PACT) in vitro and the treatment effect in vivo was investigated in this paper. Atomic force microscopy (AFM) revealed that 4i-PACT can effectively destroy membrane and wall of bacteria, resulting in leakage of its content. This was confirmed by dual fluorescent staining with acridine orange/ethidium bromide and measuring materials absorption at 260 nm. Agarose gel electrophoresis measurement showed that 4i-PACT can damage genomic DNA. Healing of wound in rat infected by mixed bacteria showed that the efficiency of 4i-PACT is dependent on the dose of light. These results showed that 4i-PACT has promising bactericidal effect both in vitro and in vivo.

引用

页数：8

共 42 条

[31] Atomic force microscopic study on morphological alterations induced by photodynamic action of Toluidine Blue O in Staphylococcus aureus and Escherichia coli [J].

Sahu, Khageswar ;

Bansal, Harsha ;

Mukherjee, Chandrachur ;

Sharma, Mrinalini ;

Gupta, Pradeep Kumar .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2009, 96 (01) :9-16

[32]

Sasagawa A., 2006, HIGH PRESS SCI TECHN, V16, P45, DOI [10.4131/jshpreview.16.45, DOI 10.4131/JSHPREVIEW.16.45]

[33] Antimicrobial, wound healing and antioxidant activity of Plagiochasma appendiculatum Lehm. et Lind [J].

Singh, Meenakshi ;

Govindarajan, Raghavan ;

Nath, Virendra ;

Rawat, Ajay Kumar Singh ;

Mehrotra, Shanta .

JOURNAL OF ETHNOPHARMACOLOGY, 2006, 107 (01) :67-72

[34]

Sperandio Felipe F, 2013, Recent Pat Antiinfect Drug Discov, V8, P108

[35] Antimicrobial properties of porphyrins [J].

Stojiljkovic, I ;

Evavold, BD ;

Kumar, V .

EXPERT OPINION ON INVESTIGATIONAL DRUGS, 2001, 10 (02) :309-320

[36] Synthesis of ZnO coated multi-walled carbon nanotubes and their antibacterial activities [J].

Sui, Minghao ;

Zhang, Lingdian ;

Sheng, Li ;

Huang, Shuhang ;

She, Lei .

SCIENCE OF THE TOTAL ENVIRONMENT, 2013, 452 :148-154

[37] Synthesis and antibacterial activity of new poly-S-lysine-porphyrin conjugates [J].

Tomé, JPC ;

Neves, MGPMS ;

Tomé, AC ;

Cavaleiro, JAS ;

Soncin, M ;

Magaraggia, M ;

Ferro, S ;

Jori, G .

JOURNAL OF MEDICINAL CHEMISTRY, 2004, 47 (26) :6649-6652

[38] Photosensitising agents - circumventing resistance and breaking down biofilms: a review [J].

Wainwright, M ;

Crossley, KB .

INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 2004, 53 (02) :119-126

[39] Photodynamic antimicrobial chemotherapy (PACT) [J].

Wainwright, M .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1998, 42 (01) :13-28

[40] 'Safe' photoantimicrobials for skin and soft-tissue infections [J].

Wainwright, Mark .

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2010, 36 (01) :14-18

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