Mechanism and In Vivo Evaluation: Photodynamic Antibacterial Chemotherapy of Lysine-Porphyrin Conjugate

被引：50

作者：

Xu, Zengping ^{[1
]}

Gao, Yuxiang ^{[2
]}

Meng, Shuai ^{[1
,3
]}

Yang, Baochen ^{[1
]}

Pang, Liyun ^{[1
]}

Wang, Chen ^{[3
]}

Liu, Tianjun ^{[1
]}

机构：

[1] Chinese Acad Med Sci, Peking Union Med Coll, Inst Biomed Engn, Tianjin Key Lab Biomed Mat, Tianjin, Peoples R China

[2] Nankai Univ, Dept Mat Chem, Tianjin 300071, Peoples R China

[3] Tianjin Med Univ, Canc Inst & Hosp, Tianjin, Peoples R China

来源：

FRONTIERS IN MICROBIOLOGY | 2016年 / 7卷

基金：

中国国家自然科学基金;

关键词：

lysine-porphyrin conjugate; photodynamic antimicrobial chemotherapy; antibiotic resistance; photosensitizer; mixed bacterial-infected wounds; GRAM-NEGATIVE BACTERIA; STAPHYLOCOCCUS-AUREUS; METHYLENE-BLUE; ANTIMICROBIAL CHEMOTHERAPY; METHICILLIN-RESISTANT; INACTIVATION; PHTHALOCYANINES; INFECTIONS; THERAPY; LIGHT;

D O I：

10.3389/fmicb.2016.00242

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Lysine-porphyrin conjugate 4i has potent photosensitive antibacterial effect on clinical isolated bacterial strains such as Methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coil, and Pseudomonas aeruginosa. The mechanism of photodynamic antibacterial chemotherapy of 4i (4i-PACT) in vitro and the treatment effect in vivo was investigated in this paper. Atomic force microscopy (AFM) revealed that 4i-PACT can effectively destroy membrane and wall of bacteria, resulting in leakage of its content. This was confirmed by dual fluorescent staining with acridine orange/ethidium bromide and measuring materials absorption at 260 nm. Agarose gel electrophoresis measurement showed that 4i-PACT can damage genomic DNA. Healing of wound in rat infected by mixed bacteria showed that the efficiency of 4i-PACT is dependent on the dose of light. These results showed that 4i-PACT has promising bactericidal effect both in vitro and in vivo.

引用

页数：8

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