L-Propionyl-carnitine as superoxide scavenger, antioxidant, and DNA cleavage protector

被引：153

作者：

Vanella, A ^{[1
]}

Russo, A ^{[1
]}

Acquaviva, R ^{[1
]}

Campisi, A ^{[1
]}

Di Giacomo, C ^{[1
]}

Sorrenti, V ^{[1
]}

Barcellona, ML ^{[1
]}

机构：

[1] Univ Catania, Inst Biochem, I-95125 Catania, Italy

来源：

CELL BIOLOGY AND TOXICOLOGY | 2000年 / 16卷 / 02期

关键词：

DNA cleavage; free radical; lipoperoxidation; L-propionylcarnitine; scavenging activity;

D O I：

10.1023/A:1007638025856

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

L-Propionylcarnitine, a propionyl ester of L-carnitine, increases the intracellular pool of L-carnitine. It exhibits a high affinity for the enzyme carnitine acetyltransferase (CAT) and, thus, is readily converted into propionyl-coenzyme A and free carnitine. It has been reported that L-propionylcarnitine possesses a protective action against heart ischemia-reperfusion injury; however, the antioxidant mechanism is not yet clear. L-Propionylcarnitine might reduce the hydroxyl radical production in the Fenton system, by chelating the iron required for the generation of hydroxyl radicals. To obtain a better insight into the antiradical mechanism of L-propionylcarnitine, the present research analyzed the superoxide scavenging capacity of L-propionylcarnitine and its effect on linoleic acid peroxidation. In addition, the effect of L-propionylcarnitine against DNA cleavage was estimated using pBR322 plasmid. We found that L-propionylcarnitine showed a dose-dependent free-radical scavenging activity. In fact, it was able to scavenge superoxide anion, to inhibit the lipoperoxidation of linoleic acid, and to protect pBR322 DNA from cleavage induced by H2O2 UV-photolysis.

引用

页码：99 / 104

页数：6

共 24 条

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