Necessity to critically review the automatic results of the Xpert Flu assay

被引：5

作者：

Engelmann, Ilka ^{[1
]}

Alidjinou, Enagnon Kazali ^{[1
]}

Lazrek, Mouna ^{[1
]}

Dewilde, Anny ^{[1
]}

Hober, Didier ^{[1
]}

机构：

[1] Univ Lille, Fac Med, CHU Lille, Lab Virol EA3610, F-59000 Lille, France

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2017年 / 88卷 / 01期

关键词：

Molecular diagnostic techniques; Flu; Influenza; Point-of-care; PCR; Discordance; INFLUENZA-A; VIRUSES; IDENTIFICATION;

D O I：

10.1016/j.diagmicrobio.2017.01.015

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

While using the Xpert Flu assay we became aware of false-negative results. The study aimed to analyze the causes of these false-negative results. One hundred fifty-nine respiratory specimens were tested in the Xpert Flu assay and in multiplex reverse transcription-polymerase chain reactions (RT-PCRs) for respiratory viruses. Discordant specimens were tested in the Influenza A/B r-gene assay. One hundred fifty-two (96%) and 151 (95%) specimens yielded concordant results for influenza A and B, respectively. Fifteen specimens tested negative in the Xpert Flu assay and positive in a multiplex RT-PCR. Positive results were confirmed for 12 of these specimens in the Influenza A/B r-gene assay. Xpert Flu assay amplification curves and endpoints suggested that the false negative results were mainly due to erroneous automatic result interpretation. We report false-negative results of the Xpert Flu assay due to erroneous automatic result interpretation. Careful analysis of amplification curves and endpoints is needed to avoid reporting of false-negative results. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：26 / 30

页数：5

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