Patterns of defence gene expression in the tomato-Verticillium interaction

In a tomato plant infected by Verticillium dahliae, race 1, compatibility or incompatibility appears to be determined in the stein, but little is known about the genes that either regulate or effect critical cellular events. In the present study, microarray and RT-PCR analyses were used to assess changes in tomato mRNA populations during both interactions. Initially, a commercially available DNA chip was used to screen gene expression at a single critical time point after inoculation of resistant and susceptible plants. From the results, the most-affected genes were selected to develop a tomato Verticillium response (TVR) DNA chip for detailed analyses of gene expression for 15 d after inoculation. Taken together, over half of the genes on the TVR array exhibited one of three distinct patterns of change, one reflecting a resistant phenotype and two being consistent with a susceptible phenotype. Of particular interest was a cluster of strongly expressed genes belonging to groups 2 and 3 that appeared to be co-ordinately down regulated in infected resistant plants relative to susceptible. Many of these genes encode pathogenesis related (PR) proteins. The data demonstrate that even though complex, the biological system can be standardized sufficiently to allow the reproducible analysis of gene expression in a whole plant system and provide patterns of transcriptional variation that can be used to assess the significance of specific genes in pathogenesis and resistance.