Downregulation of lncRNA AFAP1-AS1 by oridonin inhibits the epithelial-to-mesenchymal transition and proliferation of pancreatic cancer cells

被引：34
作者：
Lou, Songmei [1 ]
Xu, Jian [2 ]
Wang, Bili [2 ]
Li, Shuquan [2 ,3 ]
Ren, Jun [2 ]
Hu, Zhengjun [4 ]
Xu, Bin [1 ]
Luo, Feng [4 ]
机构：
[1] Zhejiang Univ, Sir Run Run Shaw Hosp, Sch Med, Dept Gen Surg, Hangzhou 310016, Zhejiang, Peoples R China
[2] Zhejiang Chinese Med Univ, Med Technol Coll, Hangzhou 310053, Zhejiang, Peoples R China
[3] Hangzhou Normal Univ, Dept Clin Lab, Affiliated Hosp, Hangzhou 310000, Zhejiang, Peoples R China
[4] Zhejiang Chinese Med Univ, Dept Clin Lab, Affiliated Hosp 1, Hangzhou 310006, Zhejiang, Peoples R China
来源：
ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2019年 / 51卷 / 08期
关键词：
oridonin; long non-coding RNA AFAP1-AS1; pancreatic cancer; epithelial-to-mesenchymal transition; EXPRESSION; APOPTOSIS; INVASION; SNAIL; SLUG;
D O I：
10.1093/abbs/gmz071
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Recent studies have demonstrated that the expression of the long non-coding RNA (lncRNA) AFAP1-AS1 in pancreatic cancer is negatively correlated with survival and prognosis. However, the effects of oridonin and lncRNA AFAP1-AS1 on the epithelial-to-mesenchymal transition (EMT) and migration of pancreatic cancer cells have not been fully elucidated. Surgery is the only potentially curative method for pancreatic cancer, but postoperative recurrence and metastasis are common. The aim of the present study was to assess the effect of oridonin and lncRNA AFAP1-AS1 silencing on pancreatic cancer cells. The pancreatic cancer cell lines BxPC-3 and PANC-1 cells were transfected with siAFAP1-AS1 and its negative control (siNC). After that, oridonin was used to treat the siAFAP1-AS1-transfected cells. The expression of lncRNA AFAP1-AS1 was downregulated in the pancreatic cancer cell lines BxPC-3 and PANC-1. The apoptosis and cell cycle progression of pancreatic cancer cells were evaluated by flow cytometry and Hoechst 33258 staining. Metastasis and invasion of BxPC-3 and PANC-1 cells were detected by transwell migration assay, real-time cell analysis, and western blot analysis. Cells were transfected with the lentiviral siAFAP1-AS1 and siNC, and tumorigenesis was evaluated in BALB/C nude mice. Immunohistochemical examination was used to verify the effects of oridonin and siAFAP1-AS1 on pancreatic cancer. The results demonstrated that the combination of oridonin and siAFAP1-AS1 inhibited pancreatic cancer cell proliferation, induced apoptosis, arrested cell cycle progression, prevented the migration, regulated EMT-related protein expression in BxPC-3 and PANC-1 cells, and inhibited pancreatic cancer cell tumorigenicity and EMT in nude mice.
引用
收藏
页码：814 / 825
页数：12
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