The microRNA miR-124 inhibits vascular smooth muscle cell proliferation by targeting S100 calcium-binding protein A4 (S100A4)

被引：45

作者：

Choe, Nakwon ^{[1
,2
]}

Kwon, Duk-Hwa ^{[1
,2
]}

Shin, Sera ^{[1
,2
]}

Kim, Yong Sook ^{[3
]}

Kim, Young-Kook ^{[2
,4
]}

Kim, Jaetaek ^{[2
,5
]}

Ahn, Youngkeun ^{[3
]}

Eom, Gwang H. ^{[1
,6
]}

Kook, Hyun ^{[1
,2
]}

机构：

[1] Chonnam Natl Univ, Med Sch, Dept Pharmacol, Gwangju 61469, South Korea

[2] Chonnam Natl Univ, Med Sch, Basic Res Lab Cardiac Remodeling, Gwangju, South Korea

[3] Chonnam Natl Univ Hosp, Dept Cardiol, Gwangju, South Korea

[4] Chonnam Natl Univ, Med Sch, Dept Biochem, Gwangju, South Korea

[5] Chung Ang Univ, Coll Med, Dept Internal Med, Div Endocrinol & Metab, Seoul, South Korea

[6] Chonnam Natl Univ, Med Sch, Med Res Ctr Gene Regulat, Gwangju, South Korea

来源：

FEBS LETTERS | 2017年 / 591卷 / 07期

基金：

新加坡国家研究基金会;

关键词：

carotid artery injury; miR-124; neointima; S100A4; vascular smooth muscle cell proliferation; ENDOTHELIAL-CELLS; DOWN-REGULATION; BREAST-CANCER; METASTASIS; MARKER; GROWTH; DIFFERENTIATION; PROGRESSION; SUPPRESSES; TRANSITION;

D O I：

10.1002/1873-3468.12606

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

S100 calcium-binding protein A4 (S100A4) induces proliferation and migration of vascular smooth muscle cells (VSMCs). We aimed to find the microRNA regulating S100A4 expression. S100A4 transcripts are abruptly increased in the acute phase of carotid arterial injury 1 day later (at day 1) but gradually decreases at days 7 and 14. Bioinformatics analysis reveals that miR-124 targets S100A4. VSMC survival is attenuated by miR-124 mimic but increased by miR-124 inhibitor. miR-124 decreases immediately after carotid arterial injury but dramatically increases at days 7 and 14. miR-124 inhibitor-induced cell proliferation is blocked by S100A4 siRNA, whereas miR-124-induced cell death is recovered by S100A4. Our findings suggest that miR-124 is a novel regulator of VSMC proliferation and may play a role in the development of neointimal proliferation.

引用

页码：1041 / 1052

页数：12

共 44 条

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