Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)

被引：15

作者：

Gao, Feng-Shan ^{[1
]}

Hu, Gui-Xue ^{[1
,2
]}

Xia, Xian-zhu ^{[3
]}

Gao, Yu-Wei ^{[3
]}

Bai, Ya-Duo ^{[2
]}

Zou, Xiao-Huan ^{[3
]}

机构：

[1] Dalian Univ, Coll Bioengn, Dept Biochem & Mol Biol, Dalian 116622, Liaoning, Peoples R China

[2] Jilin Agr Univ, Coll Anim Sci, Dept Microbiol & Immunol, Changchun 130118, Jilin, Peoples R China

[3] Acad Mil Med Sci, Inst Mil Vet Sci, Changchun 130062, Jilin, Peoples R China

来源：

JOURNAL OF VETERINARY SCIENCE | 2009年 / 10卷 / 03期

关键词：

canine coronavirus; giant panda; virus characterization; virus isolation; CONSERVATION; VIRUS; CHINA; DOGS;

D O I：

10.4142/jvs.2009.10.3.261

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo. The clinical disease profile suggested that the pandas may have suffered a viral infection. Therefore, a series of detection including virus isolation, electron microscopy, cytobiological assay, serum neutralization and RT-PCR were used to identify the virus. It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV. The results suggest that the affected pandas had been infected with CCV.

引用

页码：261 / 263

页数：3

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