Evaluation of the resistance of blood plasma to oxidative stress by oxidizability of proteins and lipids during metal-catalyzed oxidation

被引：4

作者：

Bekman, E. M. ^{[1
]}

Baranova, O. A. ^{[1
]}

Gubareva, E. V. ^{[1
]}

Shulenina, L. V. ^{[1
]}

Moskvina, S. N. ^{[1
]}

Danilogorskaya, Yu. A. ^{[1
]}

Azizova, O. A. ^{[1
]}

机构：

[1] Russian Minist Hlth, Inst Physicochem Med, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2006年 / 142卷 / 03期

关键词：

Cu2+-catalyzed oxidation of lipids and proteins; malonic dialdehyde; blood plasma; oxidative stress;

D O I：

10.1007/s10517-006-0351-5

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

A new approach for the evaluation of oxidizability of proteins and lipids in the same sample of blood plasma was proposed. We tested a method for evaluation of metal-catalyzed oxidation of fibrinogen by the formation of bityrosine cross-links during oxidation detected by the increase in fluorescence at 415 nm. A correlation was revealed between parameters of oxidizability estimated by this marker and carbonyl derivatives (dinitrophenylhydrazine method). Oxidizability of total proteins from whole plasma was compared with oxidizability of plasma lipids (marker malonic dialdehyde). Study of these parameters in patients with coronary heart disease showed that the proposed experimental approach allows us to divide the sample into several subgroups differing in the resistance to oxidative stress. These data can be used for diagnostic and prognostic purposes.

引用

页码：299 / 303

页数：5

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