A rapid and simple evaluation system for gas toxicity using luminous bacteria entrapped by a polyion complex membrane

被引：10

作者：

Komori, Kikuo ^{[1
]}

Miyajima, Shotaro ^{[1
]}

Tsuru, Tatsuro ^{[1
]}

Fujii, Takao ^{[1
]}

Mohri, Shino ^{[2
]}

Ono, Yoshiro ^{[2
]}

Sakai, Yasuyuki ^{[1
]}

机构：

[1] Univ Tokyo, Inst Ind Sci, Meguro Ku, Tokyo 1538505, Japan

[2] Okayama Univ, Dept Environm & Civil Engn, Okayama 7008530, Japan

来源：

CHEMOSPHERE | 2009年 / 77卷 / 08期

关键词：

Luminous bacteria; Gas toxicity evaluation; Environmental gas; Polyion complex; RECOMBINANT BIOLUMINESCENT BACTERIUM; SELENASTRUM-CAPRICORNUTUM; AIR POLLUTANTS; BIOSENSOR; SENSITIVITY; NAPHTHALENE; RESPONSES; EXPOSURE; GROWTH; ALGAE;

D O I：

10.1016/j.chemosphere.2009.08.004

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

We have developed a rapid and simple gas toxicity evaluation system based on bioluminescence inhibition of a marine-derived wild luminous bacterium, Vibrio fischeri. The luminous bacteria were trapped using a thin polyion complex membrane in order to allow semi direct contact between the bacteria and toxic gases. Bioluminescence inhibition ratios of the present system to six reference gases, including benzene, trichloroethylene. acetone, NO2. SO2, and CO, were evaluated, and dose-response relationships were successfully obtained after 15 min of gas exposure, except for CO gas. The sensitivity to the five gases except for CO gas of the present system was 1-3 orders of magnitude higher than that in acute animal tests. The present system also allowed for the evaluation of overall toxicity of some environmental gases containing various chemicals. These results clearly demonstrated that the present system would be a valuable prototype for rapid and on-site acute toxicity detection of a gas mixture, such as environmental gases. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1106 / 1112

页数：7

共 36 条

[1] EFFLUENT TOXICITY TESTING USING NITRIFIERS AND MICROTOX(TM) [J].