Pretreatment with heat does not affect double-strand breaks DNA rejoining in Chlamydomonas reinhardtii

被引：10

作者：

Chankova, Stephka G. ^{[1
]}

Yurina, Nadezhda P. ^{[2
]}

Dimova, Evgeniya G. ^{[1
]}

Ermohina, Olga V. ^{[2
]}

Oleskina, Yulia P. ^{[2
]}

Dimitrova, Maria T. ^{[1
]}

Bryant, Peter E. ^{[3
]}

机构：

[1] Bulgarian Acad Sci, Cent Lab Gen Ecol, BU-1113 Sofia, Bulgaria

[2] Russian Acad Sci, AN Bach Inst Biochem, Moscow 117071, Russia

[3] Univ St Andrews, Bute Med Sch, St Andrews KY16 9TS, Fife, Scotland

来源：

JOURNAL OF THERMAL BIOLOGY | 2009年 / 34卷 / 07期

基金：

俄罗斯基础研究基金会;

关键词：

Adaptive response; Chlamydomonas; DNA DSB rejoining; Gamma rays; Heat-shock proteins; ADAPTIVE RESPONSE; SACCHAROMYCES-CEREVISIAE; HYDROGEN-PEROXIDE; OXIDATIVE STRESS; SHOCK INDUCTION; CELLS; REPAIR; RADIATION; HSP70; CHLOROPLAST;

D O I：

10.1016/j.jtherbio.2009.06.002

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We aimed to clarify if heat pretreatment could protect Chlamydomonas reinhardtii cells from gamma rays DNA damaging action. It was studied whether: (1) heat pretreatment could accelerate DNA DSB rejoining; (2) chloroplast chaperones (HSP70B. HSP90C) could be involved in protection from radiation-induced DNA DSB. It was obtained that heat pretreatment (37-42 degrees C) induced minor DNA DSB levels which might be insufficient as signals for DNA DSB repair induction. No correlation between chaperones overproduction and DNA DSB rejoining was shown. These are probably the first data that HSP70B and HSP90C do not protect DNA against radiation-induced damage in a plant model system. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：332 / 336

页数：5

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